Specific DNA extraction through fluid channels with immobilization of layered double hydroxides on polycarbonate surface

Abstract

The purpose of this study was to immobilize inorganic layered double hydroxides (LDHs) on the polycarbonate (PC) substrate as the media to extract the specific DNA molecules through fluidic system and to enhance the extraction efficiency of specific DNA molecules from extreme low concentration in sample solution. LDH immobilized through solvent swelling and plasma treatment on the polymer surface captured the specific DNA molecules lysed from Escherichia coli (E. call) cells as the target DNA molecules with 2 x 10(-4) g/l of concentration in sample solution mixed biomacromolecules lysed from human blood. The encapsulated DNA molecules released through dissolve of LDHs by slight acid (pH 4-5) solution then amplified by polymerase chain reaction (PCR) process through the primers for E coli cells. The DNA molecules amplified by PCR process were characterized by gel electrophoresis to recognize the existence of E. coli cells. The results show that immobilized LDHs could be regarded as the specific DNA detector in the sample solution mixed biomacromolecules for rapid disease diagnosis through fluidic system to approach the lab-on-a-chip (LOC). (c) 2008 Elsevier B.V. All rights reserved.