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dc.contributor.authorLiu, Jr-Chien_US
dc.contributor.authorTsai, Pei-Janeen_US
dc.contributor.authorLee, Yuan C.en_US
dc.contributor.authorChen, Yu-Chieen_US
dc.date.accessioned2014-12-08T15:11:11Z-
dc.date.available2014-12-08T15:11:11Z-
dc.date.issued2008-07-15en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://dx.doi.org/10.1021/ac800487ven_US
dc.identifier.urihttp://hdl.handle.net/11536/8571-
dc.description.abstractEscherichia coli and Staphylococcus saprophyticus are the most common causes of urinary tract infections, with 80% of these infections caused by uropathogenic E. coli. Because the P fimbriae of E. coli have specificity toward Gal(alpha 1-4)Gal beta units, pigeon ovalbumin (POA), whose structure contains terminal Gal(alpha 1-4)Gal beta moieties, was used as a probe for interaction with P fimbriated E. coli. The functional affinity probes for these bacteria by immobilizing POA-a phosphoprotein-onto the surface of magnetic iron oxide nanoparticles (NPs) coated with alumina (Fe(3)O(4)@Al(2)O(3)), using the phosphate units of POA as linking groups for the formation of phosphate-alumina complexes. The immobilization process occurred within 30 s when performing the reaction under microwave heating. The magnetic POA-Fe(3)O(4)@Al(2)O(3) NPs generated using this facile approach exhibited specificity toward P fimbriated E. coli. The bacteria targeted by the affinity probes were characterized by matrix-assisted laser desorption/ionization mass spectrometry. The detection limit toward uropathogenic bacteria when using this approach was similar to 9.60 x 10(4) cfu/mL (0.5 mL).en_US
dc.language.isoen_USen_US
dc.titleAffinity capture of uropathogenic Escherichia coli using pigeon ovalbumin-bound Fe(3)O(4)@Al(2)O(3) magnetic nanoparticlesen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac800487ven_US
dc.identifier.journalANALYTICAL CHEMISTRYen_US
dc.citation.volume80en_US
dc.citation.issue14en_US
dc.citation.spage5425en_US
dc.citation.epage5432en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
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