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dc.contributor.authorSun, Y. L.en_US
dc.contributor.authorChou, Y. C.en_US
dc.contributor.authorKuan, T. C.en_US
dc.contributor.authorTu, C. F.en_US
dc.contributor.authorLin, C. S.en_US
dc.date.accessioned2014-12-08T15:11:15Z-
dc.date.available2014-12-08T15:11:15Z-
dc.date.issued2008-07-01en_US
dc.identifier.issn1065-6995en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.cellbi.2008.02.004en_US
dc.identifier.urihttp://hdl.handle.net/11536/8637-
dc.description.abstractTo express recombinant proteins in the spontaneously immortalized porcine mammary epithelial cell line (SI-PMEC) currently established in our laboratory, a chemically synthesized DNA fragment encoding the anticoagulant hirudin was used to construct a mammalian expression vector under the control of the goat beta-casein regulatory sequence. The vector, named pGB562/Hi, was transfected into the SI-PMEC cells to yield pGB562/ Hi/SI-PMEC. The pGB562/Hi/SI-PMEC cells expressed recombinant hirudin only when they were differentiated into functional structures by growth on a Matrigel-coated petri dish supplemented with the lactogenic hormone prolactin. The differentiated pGB562/Hi/SI-PMEC cells produced about 0.5-0.6 mu g of recombinant hirudin/mg of total cellular protein. These results suggest that the established SI-PMEC cells have pharmaceutical potential to inducibly express bioactive heterogeneous proteins. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectRecombinant proteinen_US
dc.subjectMammary glanden_US
dc.subjectEpithelial cellen_US
dc.subjectSI-PMECen_US
dc.subjectHirudinen_US
dc.titleExpression of recombinant anticoagulant hirudin in the differentiated cultures of the porcine mammary epithelial cell line SI-PMECen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.cellbi.2008.02.004en_US
dc.identifier.journalCELL BIOLOGY INTERNATIONALen_US
dc.citation.volume32en_US
dc.citation.issue7en_US
dc.citation.spage739en_US
dc.citation.epage747en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000260671400004-
dc.citation.woscount3-
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