標題: | Microscale MALDI Imaging of Outer-Layer Lipids in Intact Egg Chambers from Drosophila melanogaster |
作者: | Urban, Pawel L. Chang, Chia-Hsien Wu, June-Tai Chen, Yu-Chie 應用化學系 Department of Applied Chemistry |
公開日期: | 15-五月-2011 |
摘要: | Fruit fly (Drosophila melanogaster) is a standard model organism used in genetics and molecular biology. Phospholipids are building blocks of cellular membranes, and components of a complex signaling network Here, we present a facile method, based on matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), for molecular imaging of phospholipid distributions in submillimeter-sized components of the fruit fly reproductive system. Individual egg chambers were deposited on a specially prepared MALDI target comprising an aluminum slide with a rough surface created by ablation with a microsecond-laser: this helped to immobilize biological specimens, remove excess of saline solution by adhesive forces, carry out microscopic observations, and facilitated distribution of the MALDI matrix. A continuous-flow ultrasound-assisted spray was used for the deposition of MALDI matrix (9-aminoacridine) onto the sample. The upper surface of the specimen was then scanned with a 355-nm solid-state laser with a preset beam focus of 10 mu m to obtain negative-ion mode MALDI-MS images. Overall, this provided sufficient spatial resolution to reveal micrometer-scale gradient-like patterns of phospholipids along the anterior/posterior axis of egg chambers. Several phosphatidylinositols are seen to be segregated according to the number of unsaturated bonds, with an elevated abundance of polyunsaturated phosphatidylinositols within the oocyte compartment. |
URI: | http://dx.doi.org/10.1021/ac200481j http://hdl.handle.net/11536/8852 |
ISSN: | 0003-2700 |
DOI: | 10.1021/ac200481j |
期刊: | ANALYTICAL CHEMISTRY |
Volume: | 83 |
Issue: | 10 |
起始頁: | 3918 |
結束頁: | 3925 |
顯示於類別: | 期刊論文 |