完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chen, Wen-Shin | en_US |
dc.contributor.author | Yueh, Chao-Yen | en_US |
dc.contributor.author | Huang, Yung-An | en_US |
dc.contributor.author | Hwang, Eric | en_US |
dc.date.accessioned | 2014-12-08T15:11:35Z | - |
dc.date.available | 2014-12-08T15:11:35Z | - |
dc.date.issued | 2011-05-01 | en_US |
dc.identifier.issn | 0168-0102 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1016/j.neures.2011.01.002 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/8895 | - |
dc.description.abstract | Dissociated hippocampal neuron culture has long been the model system of choice for many neuroscientists. The ability to culture dissociated hippocampal neurons from genetically modified mice provides an invaluable tool for studying many neuronal processes. In this study, we established a novel method to culture dissociated hippocampal neurons from embryonic and neonatal mice. Dissociated neurons were cultured in a microchamber between the glass coverslip and the plastic cell container without the use of glial feeder cells. Our method significantly simplifies the preparation while produces healthy and long-lived neuronal cultures that are difficult to achieve without the use of feeder cells. (C) 2011 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | Mouse hippocampal neuron | en_US |
dc.subject | Primary culture | en_US |
dc.subject | Dissociated neuron culture | en_US |
dc.title | An inverted method for culturing dissociated mouse hippocampal neurons | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.neures.2011.01.002 | en_US |
dc.identifier.journal | NEUROSCIENCE RESEARCH | en_US |
dc.citation.volume | 70 | en_US |
dc.citation.issue | 1 | en_US |
dc.citation.spage | 118 | en_US |
dc.citation.epage | 123 | en_US |
dc.contributor.department | 生物科技學系 | zh_TW |
dc.contributor.department | 生物資訊及系統生物研究所 | zh_TW |
dc.contributor.department | 分子醫學與生物工程研究所 | zh_TW |
dc.contributor.department | Department of Biological Science and Technology | en_US |
dc.contributor.department | Institude of Bioinformatics and Systems Biology | en_US |
dc.contributor.department | Institute of Molecular Medicine and Bioengineering | en_US |
dc.identifier.wosnumber | WOS:000291179500018 | - |
dc.citation.woscount | 4 | - |
顯示於類別: | 期刊論文 |