完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Han, Chia-Li | en_US |
dc.contributor.author | Chen, Jinn-Shiun | en_US |
dc.contributor.author | Chan, Err-Cheng | en_US |
dc.contributor.author | Wu, Chien-Peng | en_US |
dc.contributor.author | Yu, Kun-Hsing | en_US |
dc.contributor.author | Chen, Kuei-Tien | en_US |
dc.contributor.author | Tsou, Chih-Chiang | en_US |
dc.contributor.author | Tsai, Chia-Feng | en_US |
dc.contributor.author | Chien, Chih-Wei | en_US |
dc.contributor.author | Kuo, Yung-Bin | en_US |
dc.contributor.author | Lin, Pei-Yi | en_US |
dc.contributor.author | Yu, Jau-Song | en_US |
dc.contributor.author | Hsueh, Chuen | en_US |
dc.contributor.author | Chen, Min-Chi | en_US |
dc.contributor.author | Chan, Chung-Chuan | en_US |
dc.contributor.author | Chang, Yu-Sun | en_US |
dc.contributor.author | Chen, Yu-Ju | en_US |
dc.date.accessioned | 2014-12-08T15:11:50Z | - |
dc.date.available | 2014-12-08T15:11:50Z | - |
dc.date.issued | 2011-04-01 | en_US |
dc.identifier.issn | 1535-9476 | en_US |
dc.identifier.uri | http://dx.doi.org/10.1074/mcp.M110.003087 | en_US |
dc.identifier.uri | http://hdl.handle.net/11536/9080 | - |
dc.description.abstract | We developed a multiplexed label-free quantification strategy, which integrates an efficient gel-assisted digestion protocol, high-performance liquid chromatography tandem MS analysis, and a bioinformatics alignment method to determine personalized proteomic profiles for membrane proteins in human tissues. This strategy provided accurate (6% error) and reproducible (34% relative S. D.) quantification of three independently purified membrane fractions from the same human colorectal cancer (CRC) tissue. Using CRC as a model, we constructed the personalized membrane protein atlas of paired tumor and adjacent normal tissues from 28 patients with different stages of CRC. Without fractionation, this strategy confidently quantified 856 proteins (>= 2 unique peptides) across different patients, including the first and robust detection (Mascot score: 22,074) of the well-documented CRC marker, carcinoembryonic antigen 5 by a discovery-type proteomics approach. Further validation of a panel of proteins, annexin A4, neutrophils defensin A1, and claudin 3, confirmed differential expression levels and high occurrences (48-70%) in 60 CRC patients. The most significant discovery is the overexpression of stomatin-like 2 (STOML2) for early diagnostic and prognostic potential. Increased expression of STOML2 was associated with decreased CRC-related survival; the mean survival period was 34.77 +/- 2.03 months in patients with high STOML2 expression, whereas 53.67 +/- 3.46 months was obtained for patients with low STOML2 expression. Further analysis by ELISA verified that plasma concentrations of STOML2 in early-stage CRC patients were elevated as compared with those of healthy individuals (p < 0.001), suggesting that STOML2 may be a noninvasive serological biomarker for early CRC diagnosis. The overall sensitivity of STOML2 for CRC detection was 71%, which increased to 87% when combined with CEA measurements. This study demonstrated a sensitive, label-free strategy for differential analysis of tissue membrane proteome, which may provide a roadmap for the subsequent identification of molecular target candidates of multiple cancer types. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.003087, 1-15, 2011. | en_US |
dc.language.iso | en_US | en_US |
dc.title | An Informatics-assisted Label-free Approach for Personalized Tissue Membrane Proteomics: Case Study on Colorectal Cancer | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1074/mcp.M110.003087 | en_US |
dc.identifier.journal | MOLECULAR & CELLULAR PROTEOMICS | en_US |
dc.citation.volume | 10 | en_US |
dc.citation.issue | 4 | en_US |
dc.citation.epage | en_US | |
dc.contributor.department | 生物科技學院 | zh_TW |
dc.contributor.department | College of Biological Science and Technology | en_US |
dc.identifier.wosnumber | WOS:000289067300004 | - |
dc.citation.woscount | 21 | - |
顯示於類別: | 期刊論文 |