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dc.contributor.authorCheng, Tsai-Muen_US
dc.contributor.authorMao, Simon J. T.en_US
dc.contributor.authorLai, Shiau-Tingen_US
dc.contributor.authorChang, Chia-Chingen_US
dc.contributor.authorYang, Ming-Chien_US
dc.contributor.authorChen, Nai-Chien_US
dc.contributor.authorChou, Shiu-Chingen_US
dc.contributor.authorPan, Ju-Pinen_US
dc.date.accessioned2014-12-08T15:12:04Z-
dc.date.available2014-12-08T15:12:04Z-
dc.date.issued2011-03-01en_US
dc.identifier.issn1071-5762en_US
dc.identifier.urihttp://dx.doi.org/10.3109/10715762.2010.532492en_US
dc.identifier.urihttp://hdl.handle.net/11536/9260-
dc.description.abstractPatients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H(2)O(2) generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN(3), a peroxidase inhibitor, catalase, a H(2)O(2) decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H(2)O(2) production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN(3) and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.en_US
dc.language.isoen_USen_US
dc.titleHaemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H(2)O(2) generation from polyunsaturated fatty acidsen_US
dc.typeArticleen_US
dc.identifier.doi10.3109/10715762.2010.532492en_US
dc.identifier.journalFREE RADICAL RESEARCHen_US
dc.citation.volume45en_US
dc.citation.issue3en_US
dc.citation.spage303en_US
dc.citation.epage316en_US
dc.contributor.department生物科技學院zh_TW
dc.contributor.departmentCollege of Biological Science and Technologyen_US
Appears in Collections:Articles