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dc.contributor.authorLee, I-Liangen_US
dc.contributor.authorLi, Pei-Shanen_US
dc.contributor.authorYu, Wei-Linen_US
dc.contributor.authorShen, Hsin-Hsinen_US
dc.date.accessioned2014-12-08T15:12:05Z-
dc.date.available2014-12-08T15:12:05Z-
dc.date.issued2011-03-01en_US
dc.identifier.issn1046-5928en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.pep.2010.08.014en_US
dc.identifier.urihttp://hdl.handle.net/11536/9266-
dc.description.abstractHuman vascular endothelial growth factor isoform 165 (VEGF165) is the first known member belonging to the VEGF protein family that plays a critical role in new blood vessel formation in vivo. This study presents a new protocol with optimized conditions for rapidly producing untagged recombinant and biological active human VEGF165 (rhVEGF165) using Escherichia coli cells. Protein was isolated from inclusion bodies, purified by gel filtration and ion exchange chromatography, and subjected to protein refolding and renaturation. The biological activity of rhVEGF165 is comparable with VEFG from eukaryotic source according to human umbilical vein endothelial cells (HUVEC) proliferation assay. Therefore, the present procedures provide a fast and easy way to produce this therapeutic protein. (C) 2010 Published by Elsevier Inc.en_US
dc.language.isoen_USen_US
dc.subjectExpressionen_US
dc.subjectPurificationen_US
dc.subjectRefoldingen_US
dc.subjectRecombinant VEGF proteinen_US
dc.subjectAngiogenesisen_US
dc.subjectUntagged proteinen_US
dc.titleProkaryotic expression, refolding, and purification of functional human vascular endothelial growth factor isoform 165: Purification procedures and refolding conditions revisiteden_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.pep.2010.08.014en_US
dc.identifier.journalPROTEIN EXPRESSION AND PURIFICATIONen_US
dc.citation.volume76en_US
dc.citation.issue1en_US
dc.citation.spage54en_US
dc.citation.epage58en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000286170900007-
dc.citation.woscount1-
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