Full metadata record
DC FieldValueLanguage
dc.contributor.authorPai, Chien-Chunen_US
dc.contributor.authorKuo, Tzong-Fuen_US
dc.contributor.authorMao, Simon J. T.en_US
dc.contributor.authorChuang, Tien-Fuen_US
dc.contributor.authorLin, Chen-Sien_US
dc.contributor.authorChu, Rea-Minen_US
dc.date.accessioned2014-12-08T15:12:08Z-
dc.date.available2014-12-08T15:12:08Z-
dc.date.issued2011-02-15en_US
dc.identifier.issn0165-2427en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.vetimm.2010.10.013en_US
dc.identifier.urihttp://hdl.handle.net/11536/9303-
dc.description.abstractCanine transmissible venereal tumor (CTVT) is a naturally occurring tumor that can be transmitted between dogs via live tumor cell inoculation. It is also a spontaneous self-regression tumor and its behavior is closely related to host immune responses. Since CTVT had been widely used for tumor models in canine cancers, whether this self-regression may overtake the immunity elicited from an exogenous tumor vaccine remains unclear and certainly worthwhile to be investigated. In this study, we used DCs/tumor hybrids as a tumor vaccine to evaluate the CTVT model. We prepared mature allogeneic dendritic cells from bone marrow and then assessed their phenotype (CD80, CD83, CD86, CD1a, CD11c, CD40 and MHC II), antigen uptake and presenting abilities. Fused dendritic cell/CTVT hybrids were then used as a vaccine, administered three times at two-week intervals via subcutaneous injection near the bilateral auxiliary and inguinal lymph nodes. In comparison with unvaccinated dogs (spontaneous regressed group), within a period of 2.5 months, the vaccinations substantially inhibited tumor progression (p<0.05) and accelerated the rate of regression by a mechanism involving amplification of the host tumor-specific adaptive immune responses and NK cytotoxicity (p<0.001). Pathologic examination revealed early massive lymphocyte infiltration resulting in final tumor necrosis. In addition, there are not any detectable effects on routine physical, body temperature or blood chemistry examinations. In conclusion, our data furnishes a reference value showing that CTVT is a model of potential use for the study of immunity elicited by vaccines against tumors, and also enable early-phase evaluation of the dendritic cell/tumor vaccine in terms of raising host immunity. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectCanine transmissible venereal tumor (CTVT)en_US
dc.subjectBone-marrow derived dendritic cells (BMDCs)en_US
dc.subjectTumor vaccineen_US
dc.titleImmunopathogenic behaviors of canine transmissible venereal tumor in dogs following an immunotherapy using dendritic/tumor cell hybriden_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.vetimm.2010.10.013en_US
dc.identifier.journalVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGYen_US
dc.citation.volume139en_US
dc.citation.issue2-4en_US
dc.citation.spage187en_US
dc.citation.epage199en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000287110800013-
dc.citation.woscount5-
Appears in Collections:Articles


Files in This Item:

  1. 000287110800013.pdf

If it is a zip file, please download the file and unzip it, then open index.html in a browser to view the full text content.