標題: | Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions |
作者: | Liu, Kuo-Liang Wu, Chien-Chen Huang, Ying-Jung Peng, Hwei-Ling Chang, Hwan-You Chang, Pin Hsu, Long Yew, Tri-Rung 生物科技學系 電子物理學系 Department of Biological Science and Technology Department of Electrophysics |
公開日期: | 2008 |
摘要: | A novel and disposable microchip (K-kit) with SiO(2) nano-membranes was developed and used as a specimen kit for in situ imaging of living organisms in an aqueous condition using transmission electron microscopy (TEM) without equipment modi. cation. This K-kit enabled the successful TEM observation of living Escherichia coli cells and the tellurite reduction process in Klebsiella pneumoniae. The K. pneumoniae and Saccharomyces cerevisiae can stay alive in K-kit after continuous TEM imaging for up to 14 s and 42 s, respectively. Besides, different tellurite reduction profiles in cells grown in aerobic and anaerobic environments can be clearly revealed. These results demonstrate that the K-kit developed in this paper can be useful for observing living organisms and monitoring biological processes in situ. |
URI: | http://hdl.handle.net/11536/9791 http://dx.doi.org/10.1039/b804986f |
ISSN: | 1473-0197 |
DOI: | 10.1039/b804986f |
期刊: | LAB ON A CHIP |
Volume: | 8 |
Issue: | 11 |
起始頁: | 1915 |
結束頁: | 1921 |
顯示於類別: | 期刊論文 |