標題: Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions
作者: Liu, Kuo-Liang
Wu, Chien-Chen
Huang, Ying-Jung
Peng, Hwei-Ling
Chang, Hwan-You
Chang, Pin
Hsu, Long
Yew, Tri-Rung
生物科技學系
電子物理學系
Department of Biological Science and Technology
Department of Electrophysics
公開日期: 2008
摘要: A novel and disposable microchip (K-kit) with SiO(2) nano-membranes was developed and used as a specimen kit for in situ imaging of living organisms in an aqueous condition using transmission electron microscopy (TEM) without equipment modi. cation. This K-kit enabled the successful TEM observation of living Escherichia coli cells and the tellurite reduction process in Klebsiella pneumoniae. The K. pneumoniae and Saccharomyces cerevisiae can stay alive in K-kit after continuous TEM imaging for up to 14 s and 42 s, respectively. Besides, different tellurite reduction profiles in cells grown in aerobic and anaerobic environments can be clearly revealed. These results demonstrate that the K-kit developed in this paper can be useful for observing living organisms and monitoring biological processes in situ.
URI: http://hdl.handle.net/11536/9791
http://dx.doi.org/10.1039/b804986f
ISSN: 1473-0197
DOI: 10.1039/b804986f
期刊: LAB ON A CHIP
Volume: 8
Issue: 11
起始頁: 1915
結束頁: 1921
顯示於類別:期刊論文


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