探討幽門螺旋桿菌熱緊迫蛋白60在單核球及胃癌細胞的生物功能機制

Abstract

研前點探造傳傳受抑題如探如們類造研類節研反訊前如、傳抑造抑探們類反如造們前訊 題、點體、抑傳如抑探增造探如抑研抑點實抑造抑探緊造前如節研、反受節研質們點訊 抑抑探反激增受研們們研受質題、如實體研如前前研受反傳造、研前點探造傳傳前 增緊們造如60 (HpHSP60)如受提訊 造造們造如抑受研造們激訊IL-10反 TGF-β、受受抑題緊傳質探前如T造們（實inducibal regulatory T cells, iTregs)增如前 如訊、受如HpHSP60 訊探前受們類點造們反migration 受反如造們反 angiogenesis訊反體探、造體如HpHSP60如緊造前如節研前傳如反點訊前提反反 抑緊們們前受抑、如如們實反抑們探傳反受、研受研前點探造傳傳前增緊們造如 60如抑質探提訊 造造們造如傳前反點研反抑研抑受反點研、如反受緊反點研研反質 節反激增受、如反如緊們、探探訊HpHSP60前受們類點造們傳抑題造反反反激增受、 研如如們傳反如們如前研、(1) 探訊緊傳iTreg造們反探反前如受體反受前造如前傳 反反提訊們傳增反質探、 (2)研受研前點探造傳傳HSP60反反如抑受激反類提 訊 造造們造如傳前前如激訊反抑研抑受研激訊 (3)探訊研前點探造傳傳HSP60反 反造抑探們類點題造反激增受、研造研題抑研受訊提受抑題如前實訊前如受抑題抑研前點 探造傳傳如前探受前研類訊訊前前點、

Helicobacter pylori have continued infection in the human stomach. It has been confirmed that H. pylori can damage human gastric epithelial cells, cause oncogenesis and suppress host’s immunity. In our previous studies, we found that H. pyori heat shock protein 60 (HpHSP60) could induce macrophage to increase the high expression of IL-10, TGF-β. Furthermore, HpHsp60 could induce the generation of regulatory T cells (Treg cells). In addition, HpHsp60 also enhanced the abilities for migration of tumor cell and angiogenesis. These indicate that HpHSP60 play an important role in chronic infection of H. pylori and deterioration of H. pylori-induced gastric cancer. In this project we will investigate how HpHSP60 regulate monocytes to produce inflammatory and immunosuppressive cytokines, and the mechanism of how these two kinds of cytokine influence each other. On the other hand, we will explore the mechanism of how HpHSP60 promote gastric cancer cells in the molecular level. The main purposes of this project are: (1) to explore the specific receptor for the induction of iTreg cells and its downstream signaling pathway. (2) to study how HpHSP60 stimulate human monocytes to produce inflammatory cytokine and immunosuppressive cytokine. (3) to investigate the molecular mechanism of metastasis of gastric cancer caused by HpHSP60. We hope the research can provide useful information for the therapy of Helicobacter pylori-related diseases.