Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7
| dc.citation.epage | 565 | en_US |
| dc.citation.issue | 4 | en_US |
| dc.citation.spage | 558 | en_US |
| dc.citation.volume | 24 | en_US |
| dc.citation.woscount | 27 | |
| dc.contributor.author | Liu, Yi-Ju | en_US |
| dc.contributor.author | Yao, Da-Jeng | en_US |
| dc.contributor.author | Chang, Hwan-You | en_US |
| dc.contributor.author | Liu, Chien-Ming | en_US |
| dc.contributor.author | Chen, Chih | en_US |
| dc.contributor.department | 材料科學與工程學系 | zh_TW |
| dc.contributor.department | Department of Materials Science and Engineering | en_US |
| dc.date.accessioned | 2014-12-08T15:10:35Z | |
| dc.date.available | 2014-12-08T15:10:35Z | |
| dc.date.issued | 2008-12-01 | en_US |
| dc.description.abstract | This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis. (C) 2008 Elsevier B.V. All rights reserved. | en_US |
| dc.identifier.doi | 10.1016/j.bios.2008.06.019 | en_US |
| dc.identifier.issn | 0956-5663 | en_US |
| dc.identifier.journal | BIOSENSORS & BIOELECTRONICS | en_US |
| dc.identifier.uri | http://dx.doi.org/10.1016/j.bios.2008.06.019 | en_US |
| dc.identifier.uri | https://ir.lib.nycu.edu.tw/handle/11536/8094 | |
| dc.identifier.wosnumber | WOS:000261262000009 | |
| dc.language.iso | en_US | en_US |
| dc.subject | DNA detection | en_US |
| dc.subject | Magnetic bead | en_US |
| dc.subject | Quantum dots | en_US |
| dc.subject | Biotin-streptavidin | en_US |
| dc.subject | Biotinlyted linker | en_US |
| dc.title | Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7 | en_US |
| dc.type | Article | en_US |