Differential Stereocontrolled Formation of Tricyclic Triterpenes by Mutation of Tyrosine 99 of the Oxidosqualene-Lanosterol Cyclase from Saccharomyces cerevisiae
| dc.citation.epage | 5737 | en_US |
| dc.citation.issue | 33 | en_US |
| dc.citation.spage | 5731 | en_US |
| dc.citation.volume | en_US | |
| dc.citation.woscount | 9 | |
| dc.contributor.author | Wu, Tung-Kung | en_US |
| dc.contributor.author | Li, Wen-Hsuan | en_US |
| dc.contributor.author | Chang, Cheng-Hsiang | en_US |
| dc.contributor.author | Wen, Hao-Yu | en_US |
| dc.contributor.author | Liu, Yuan-Ting | en_US |
| dc.contributor.author | Chang, Yi-Chun | en_US |
| dc.contributor.department | 生物科技學系 | zh_TW |
| dc.contributor.department | Department of Biological Science and Technology | en_US |
| dc.date.accessioned | 2014-12-08T15:08:25Z | |
| dc.date.available | 2014-12-08T15:08:25Z | |
| dc.date.issued | 2009-11-01 | en_US |
| dc.description.abstract | The function of the Tyr99 residue from Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase (ERG7) was analyzed by constructing deletion and site-saturated mutants. Two truncated intermediates, (13 alpha H)-isomalabarica-14Z,17E,21-trien-3 beta-ol and (13 alpha H)-isomalabarica-14E,17E,21-trien-3 beta-ol, were isolated from the ERG7(Y99X) mutants. These results suggest that the functional role of ERG7(Y99) is to affect both chair-boat 6-6-5 tricyclic Markovnikov C-14 cation stabilization and the stereochemistry of the protons at the C-15 position for subsequent deprotonation. ((C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009) | en_US |
| dc.identifier.doi | 10.1002/ejoc.200900638 | en_US |
| dc.identifier.issn | 1434-193X | en_US |
| dc.identifier.journal | EUROPEAN JOURNAL OF ORGANIC CHEMISTRY | en_US |
| dc.identifier.uri | http://dx.doi.org/10.1002/ejoc.200900638 | en_US |
| dc.identifier.uri | https://ir.lib.nycu.edu.tw/handle/11536/6510 | |
| dc.identifier.wosnumber | WOS:000272330600005 | |
| dc.language.iso | en_US | en_US |
| dc.subject | Mutagenesis | en_US |
| dc.subject | Cyclization | en_US |
| dc.subject | Enzymes | en_US |
| dc.subject | Reaction mechanisms | en_US |
| dc.subject | Terpenoids | en_US |
| dc.title | Differential Stereocontrolled Formation of Tricyclic Triterpenes by Mutation of Tyrosine 99 of the Oxidosqualene-Lanosterol Cyclase from Saccharomyces cerevisiae | en_US |
| dc.type | Article | en_US |