Expression of recombinant anticoagulant hirudin in the differentiated cultures of the porcine mammary epithelial cell line SI-PMEC
| dc.citation.epage | 747 | en_US |
| dc.citation.issue | 7 | en_US |
| dc.citation.spage | 739 | en_US |
| dc.citation.volume | 32 | en_US |
| dc.citation.woscount | 3 | |
| dc.contributor.author | Sun, Y. L. | en_US |
| dc.contributor.author | Chou, Y. C. | en_US |
| dc.contributor.author | Kuan, T. C. | en_US |
| dc.contributor.author | Tu, C. F. | en_US |
| dc.contributor.author | Lin, C. S. | en_US |
| dc.contributor.department | 生物科技學系 | zh_TW |
| dc.contributor.department | Department of Biological Science and Technology | en_US |
| dc.date.accessioned | 2014-12-08T15:11:15Z | |
| dc.date.available | 2014-12-08T15:11:15Z | |
| dc.date.issued | 2008-07-01 | en_US |
| dc.description.abstract | To express recombinant proteins in the spontaneously immortalized porcine mammary epithelial cell line (SI-PMEC) currently established in our laboratory, a chemically synthesized DNA fragment encoding the anticoagulant hirudin was used to construct a mammalian expression vector under the control of the goat beta-casein regulatory sequence. The vector, named pGB562/Hi, was transfected into the SI-PMEC cells to yield pGB562/ Hi/SI-PMEC. The pGB562/Hi/SI-PMEC cells expressed recombinant hirudin only when they were differentiated into functional structures by growth on a Matrigel-coated petri dish supplemented with the lactogenic hormone prolactin. The differentiated pGB562/Hi/SI-PMEC cells produced about 0.5-0.6 mu g of recombinant hirudin/mg of total cellular protein. These results suggest that the established SI-PMEC cells have pharmaceutical potential to inducibly express bioactive heterogeneous proteins. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved. | en_US |
| dc.identifier.doi | 10.1016/j.cellbi.2008.02.004 | en_US |
| dc.identifier.issn | 1065-6995 | en_US |
| dc.identifier.journal | CELL BIOLOGY INTERNATIONAL | en_US |
| dc.identifier.uri | http://dx.doi.org/10.1016/j.cellbi.2008.02.004 | en_US |
| dc.identifier.uri | https://ir.lib.nycu.edu.tw/handle/11536/8637 | |
| dc.identifier.wosnumber | WOS:000260671400004 | |
| dc.language.iso | en_US | en_US |
| dc.subject | Recombinant protein | en_US |
| dc.subject | Mammary gland | en_US |
| dc.subject | Epithelial cell | en_US |
| dc.subject | SI-PMEC | en_US |
| dc.subject | Hirudin | en_US |
| dc.title | Expression of recombinant anticoagulant hirudin in the differentiated cultures of the porcine mammary epithelial cell line SI-PMEC | en_US |
| dc.type | Article | en_US |