Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping
| dc.citation.epage | 759 | en_US |
| dc.citation.issue | 4 | en_US |
| dc.citation.spage | 756 | en_US |
| dc.citation.volume | 15 | en_US |
| dc.citation.woscount | 8 | |
| dc.contributor.author | Yeh, Hsu-Hua | en_US |
| dc.contributor.author | Chang, Shu-Lin | en_US |
| dc.contributor.author | Chiang, Yi-Ming | en_US |
| dc.contributor.author | Bruno, Kenneth S. | en_US |
| dc.contributor.author | Oakley, Berl R. | en_US |
| dc.contributor.author | Wu, Tung-Kung | en_US |
| dc.contributor.author | Wang, Clay C. C. | en_US |
| dc.contributor.department | 生物科技學系 | zh_TW |
| dc.contributor.department | Department of Biological Science and Technology | en_US |
| dc.date.accessioned | 2014-12-08T15:29:54Z | |
| dc.date.available | 2014-12-08T15:29:54Z | |
| dc.date.issued | 2013-02-15 | en_US |
| dc.description.abstract | We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA In A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbA Delta R+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes. | en_US |
| dc.identifier.doi | 10.1021/ol303328t | en_US |
| dc.identifier.issn | 1523-7060 | en_US |
| dc.identifier.journal | ORGANIC LETTERS | en_US |
| dc.identifier.uri | http://dx.doi.org/10.1021/ol303328t | en_US |
| dc.identifier.uri | https://ir.lib.nycu.edu.tw/handle/11536/21435 | |
| dc.identifier.wosnumber | WOS:000315254500010 | |
| dc.language.iso | en_US | en_US |
| dc.title | Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping | en_US |
| dc.type | Article | en_US |