标题: | 发展一套超高灵敏度且即时检测流感病毒的奈米线场效电晶体生物感测器(I) Development of an Ultrasensitive and Real-Time Sensing Nanowire-Field Effect Transistor Biosensor for Influenza Virus (I) |
作者: | 杨裕雄 YANG YUH-SHYONG 国立交通大学生物科技学系(所) |
关键字: | 半导体元件;奈米线场效电晶体;禽流感病毒;生物感测器;Semiconductor device;nanowire field effect transistor;avian influenza virus;biosensor |
公开日期: | 2008 |
摘要: | 本计画之研究重点为结合分子检测技术及奈米半导体元件,以开发一套具有超高灵敏度、无须标 的、可即时侦测、操作简便的奈米线场效电晶体生物感测器。使用多晶矽奈米线场效电晶体为本计画 之特色,由于其制备过程相容于现今商业化之半导体制程,研发完成之产品,将可结合台湾现有的半 导体技术来大量生产。我们将以禽流感病毒的RNA 序列及表面蛋白为侦测标的,将相对应之DNA 探针或单株抗体分别固定化于矽奈米线表面,在侦测标的物附着于奈米线表面时,奈米线场效电晶体 之电性将产生极为灵敏的变化,藉此得到高灵敏度与专一性之检测。我们将先选择几种不同亚型(将 先以H5 与H7 为主)的禽流感病毒为测试目标,分别完成单独的可行性测试,并进行临床测试。同时, 将藉由改善元件特性及奈米线表面生物分子修饰效率,提高感测灵敏度与专一性。未来将开发成微阵 列及可携式奈米线场效电晶体生物感测器,作为未来商业产品开发之基础。 We propose the use of semiconductor device as transducer for molecular diagnosis to develop an ultrahigh sensitivity, label-free, real time detection and easy to use biosensing system using silicon nanowire field effect transistor (SNW FET). One of the unique features of this project is to use poly silicon nanowire field transistor (poly-SNW FET) for its fabrication is compatible with current commercial semiconductor process. It is important for the future products will rely on the availability of the SNW FET. We will immobilize DNA or antibody, which is complementary to influenza virus RNA or against influenza virus’ surface protein, respectively, on the nanowire surface of SNW FET. The high sensitive and high specificity diagnosis will be achieved when the complementary RNA or antigen protein interacts on the nanowire surface of SNW FET. The electronic response of SNW FET is very sensitive to the variation of charges on its surface. Avian influenza viruses will be our primary targets especially for H5 and H7 subtypes. In vitro test in the laboratory will be established by following the clinical test in collaboration with medical center. We will concurrently improve the sensing sensitivity through the fabrication of semiconductor chip and through the biomolecular modification of the nanowire surface. In the future, a portable diagnostic instrument with micro array SNW FET chip will be explored as the basis for commercial product development. |
官方说明文件#: | NSC97-2321-B009-001 |
URI: | http://hdl.handle.net/11536/102352 https://www.grb.gov.tw/search/planDetail?id=1667531&docId=286444 |
显示于类别: | Research Plans |