雷射捕陷結晶法之研究(I)

Abstract

在 2007 年我們首次利用近紅外光之連續式雷射光聚焦於溶液中，雷射 光壓可施於溶液中之奈米分子聚合物上，而使聚合物在焦點附近聚合，最 後形成結晶；此現象我們將其命名為「雷射捕陷結晶法」(Laser Trapping Crystallization)。吾人將在交通大學建立這種「雷射捕陷結晶法」技術，並 將試圖瞭解這種雷射引致之嶄新現象的產生機構及分子動態學，例如：何 種分子組合較具優勢？其大小及尺寸分布如何？它們如何成核？為了能 夠精確控制雷射捕陷及結晶，吾人必須探求最合適的光學條件以及分子濃 度及溶液種類，並且瞭解其理論依據。吾人將嘗試各種不同分子結晶的機 構及分子動態學，而最終目的是產生蛋白質結晶。

The photon pressure is exerted on nm-sized molecular aggregates in solution by focusing a near infrared CW laser beam, and the aggregates are gathered at the focal point. Eventually they may form crystal, which we demonstrated for the first time in 2007 and named “Laser Trapping Crystallization”. In NCTU, “Laser Trapping Crystallization” will be established as a general crystallization method in NCTU. We will elucidate underlying molecular dynamics and mechanism of this new laser-induced phenomenon. What kinds of molecular assemblies are preferred, how much is their size, how they fluctuate, and how they become nucleus? To control trapping and crystallization, suitable optical conditions and nice combinations of molecules and solvents should be searched and theoretically interpreted. Various molecules will be examined for molecular dynamic and mechanism studies and we will try to crystallize proteins.