標題: | Selective enrichment of ochratoxin A using human serum albumin bound magnetic beads as the concentrating probes for capillary electrophoresis/electrospray ionization-mass spectrometric analysis |
作者: | Hong, Chen-Yu Chen, Yu-Chie 應用化學系 應用化學系分子科學碩博班 Department of Applied Chemistry Institute of Molecular science |
關鍵字: | CE-ESI-MS;albumin;magnetic beads;ochratoxin A |
公開日期: | 3-Aug-2007 |
摘要: | Ochratoxin A (OTA) is a toxicant commonly present in many food products. Conventionally, immuno-affinity analysis is applied to rapidly screen the presence of OTA in food. However, antibodies are expensive. In this study, we present a new approach for selectively enriching OTA from aqueous samples using human serum albumin (HSA) bound magnetic beads as the affinity probes, followed by the analysis of CE/ESI-MS. In addition to demonstrating the feasibility of using the affinity probes to concentrate OTA, we also propose a rapid concentration and elution method for extraction, that is, OTA are extracted from aqueous samples by pipetting the samples in and out of a sample vial for 1 min followed by elution with pipetting for another minute. On the basis of the magnetic property, the affinity magnetic probe-target species could be rapidly isolated from the solution during the process of extraction and elution by magnetic separation. CE/ESI-MS, coupled by the electrodeless/sheathless interface, is used for the analysis of the samples. As this method features speed and cost-effectiveness, it is suitable for the purpose of rapid screening. In fact, the lowest detection limit for OTA is similar to 4 x 10(-3) mg/L. (c) 2007 Elsevier B.V. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.chroma.2007.05.026 http://hdl.handle.net/11536/10452 |
ISSN: | 0021-9673 |
DOI: | 10.1016/j.chroma.2007.05.026 |
期刊: | JOURNAL OF CHROMATOGRAPHY A |
Volume: | 1159 |
Issue: | 1-2 |
起始頁: | 250 |
結束頁: | 255 |
Appears in Collections: | Articles |
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