标题: | METHOD OF PROTEIN PURIFICATION |
作者: | LI Yaw-Kuen WU Yue-Jin |
公开日期: | 30-一月-2014 |
摘要: | A method for producing a target protein is provided, which includes steps described below. A crude extract including a fusion protein is provided. The fusion protein includes a tag, a target protein and a linker inserted between the tag and the target protein. The fusion protein and magnetic particles are then bound to form a magnetic particle-binding fusion protein. Finally, the linker of the magnetic particle-binding fusion protein undergoes autocleavage by using a cleavage buffer solution to release the target protein. A one-pot process for producing a purified target protein is also provided. |
官方说明文件#: | C07K001/14 |
URI: | http://hdl.handle.net/11536/104958 |
专利国: | USA |
专利号码: | 20140031531 |
显示于类别: | Patents |
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