标题: METHOD OF PROTEIN PURIFICATION
作者: LI Yaw-Kuen
WU Yue-Jin
公开日期: 30-一月-2014
摘要: A method for producing a target protein is provided, which includes steps described below. A crude extract including a fusion protein is provided. The fusion protein includes a tag, a target protein and a linker inserted between the tag and the target protein. The fusion protein and magnetic particles are then bound to form a magnetic particle-binding fusion protein. Finally, the linker of the magnetic particle-binding fusion protein undergoes autocleavage by using a cleavage buffer solution to release the target protein. A one-pot process for producing a purified target protein is also provided.
官方说明文件#: C07K001/14
URI: http://hdl.handle.net/11536/104958
专利国: USA
专利号码: 20140031531
显示于类别:Patents


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  1. 20140031531.pdf

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