標題: | Thea sinensis melanin prevents cisplatin-induced nephrotoxicity in mice |
作者: | Hung, Yao-Ching Huang, G. Steven Lin, Li-Wei Hong, Meng-Yen Se, Pei-San 材料科學與工程學系奈米科技碩博班 Graduate Program of Nanotechnology , Department of Materials Science and Engineering |
關鍵字: | Theo sinensis melanin;cisplatin;nephrotoxicity;real-time RT-PCR |
公開日期: | 1-Jul-2007 |
摘要: | The preventive effect of Thea sinensis melanin (TSM) against cisplatin-induced nephrotoxicity was studied on ICR mice. Animals were given 20 mg/kg i.p. of cisplatin, and TSM was injected i.p. in doses 10-40 mg/kg 2 h before intoxication. The protective effects were evidenced by a complete inhibition of the cisplatin-induced elevation of serum Blood Urea nitrogen (BUN), prevention of oxidative stress, and complete blockade of cisplatin-induced elevation of serum creatinine. TSM by itself, however, did not affect the renal functional parameters, including serum BUN and creatinine. Real-time RT-PCR was applied to quantify mRNA levels of cisplatin-treated mouse kidney compared to normal mouse kidney for selected marker genes. Cisplatin treatment increases mRNA levels 40-fold for glutathione-S-transferases (Gstp2), 15-fold for soluble epoxide hydrolase (Ephx1), 15-fold for lipocalin 2 (Lcn2), 9-fold for lysozyme (Lyz), 5-fold for UDP glycosyltransferase 2 (Utg2b), 30-fold for survival motor neuron (Smn1), 30-fold for guanidinoacetate methyltransferase (Gamt), 80-fold for urine retinol binding protein (Rbp4), 60-fold for aminopeptidase N (Apn), 60-fold for cytochrome P450 (Cyp2d18), and 100-fold for ornithine aminotransferase (Oat). Pre-administration of TSM restored normal expression of marker genes for cisplatin-treated mouse kidneys. TSM by itself, however, did not affect the transcription for marker genes. Results obtained demonstrate that TSM pre-administration can prevent the renal toxic effects of cisplatin. (c) 2007 Elsevier Ltd. All rights reserved. |
URI: | http://dx.doi.org/10.1016/j.fct.2006.12.017 http://hdl.handle.net/11536/10610 |
ISSN: | 0278-6915 |
DOI: | 10.1016/j.fct.2006.12.017 |
期刊: | FOOD AND CHEMICAL TOXICOLOGY |
Volume: | 45 |
Issue: | 7 |
起始頁: | 1123 |
結束頁: | 1130 |
Appears in Collections: | Articles |
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