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dc.contributor.authorLo, Chun-Yuenen_US
dc.contributor.authorChen, Wei-Yuen_US
dc.contributor.authorChen, Cheng-Taien_US
dc.contributor.authorChen, Yu-Chieen_US
dc.date.accessioned2014-12-08T15:14:46Z-
dc.date.available2014-12-08T15:14:46Z-
dc.date.issued2007en_US
dc.identifier.issn1535-3893en_US
dc.identifier.urihttp://hdl.handle.net/11536/11153-
dc.identifier.urihttp://dx.doi.org/10.1021/pr060333gen_US
dc.description.abstractIron oxide nanocomposites of magnetic particles coated with zirconia were used as affinity probes to selectively concentrate phosphopeptides from tryptic digests of alpha- and beta-caseins, milk, and egg white to exemplify the enrichment of phosphopeptides from complex samples. Phosphopeptides, in quantities sufficient for characterization by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), were enriched by the affinity probes within only 30 s. The affinity probe-target species conjugates were separated from the sample solution simply by applying an external magnetic field. The detection limit for tryptic digest of beta-casein using this approach is similar to 45 fmol. Furthermore, we combined this enrichment method with a rapid enzymatic digestion method, that is, microwave-assisted enzymatic digestion using magnetic particles as the microwave absorbers, to speed up the tryptic digest reactions. Thus, we alternatively enriched phosphoproteins on the zirconia-coated particles followed by mixing with trypsin and heated the mixture in a microwave oven for 1 min. The particles remaining in the mixture were used as affinity probes to selectively enrich phosphopeptides from the tryptic digestion product by pipeting, followed by characterization using MALDI MS. Using the bifunctional zirconia-coated magnetic particles as both the affinity probes and the microwave absorbers could greatly reduce the time for the purification and characterization of phosphopeptides from complex samples.en_US
dc.language.isoen_USen_US
dc.subjectzirconiaen_US
dc.subjectiron oxideen_US
dc.subjectmagnetic particlesen_US
dc.subjectphosphopeptidesen_US
dc.subjectMALDI MSen_US
dc.subjectconcentrating probesen_US
dc.titleRapid enrichment of phosphopeptides from tryptic digests of proteins using iron oxide nanocomposites of magnetic particles coated with zirconia as the concentrating probesen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/pr060333gen_US
dc.identifier.journalJOURNAL OF PROTEOME RESEARCHen_US
dc.citation.volume6en_US
dc.citation.issue2en_US
dc.citation.spage887en_US
dc.citation.epage893en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000243927900045-
dc.citation.woscount96-
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