標題: Optical trapping of a spherically symmetric sphere in the ray-optics regime: a model for optical tweezers upon cells
作者: Chang, YR
Hsu, L
Chi, S
電子物理學系
光電工程學系
Department of Electrophysics
Department of Photonics
公開日期: 1-Jun-2006
摘要: Since their invention in 1986, optical tweezers have become a popular manipulation and force measurement tool in cellular and molecular biology. However, until recently there has not been a sophisticated model for optical tweezers on trapping cells in the ray-optics regime. We present a model for optical tweezers to calculate the optical force upon a spherically symmetric multilayer sphere representing a common biological cell. A numerical simulation of this model shows that not only is the magnitude of the optical force upon a Chinese hamster ovary cell significantly three times smaller than that upon a polystyrene bead of the same size, but the distribution of the optical force upon a cell is also much different from that upon a uniform particle, and there is a 30% difference in the optical trapping stiffness of these two cases. Furthermore, under a small variant condition for the refractive indices of any adjacent layers of the sphere, this model provides a simple approximation to calculate the optical force and the stiffness of an optical tweezers system. (c) 2006 Optical Society of America.
URI: http://dx.doi.org/10.1364/AO.45.003885
http://hdl.handle.net/11536/12187
ISSN: 0003-6935
DOI: 10.1364/AO.45.003885
期刊: APPLIED OPTICS
Volume: 45
Issue: 16
起始頁: 3885
結束頁: 3892
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