標題: Potent Antibacterial Nanoparticles for Pathogenic Bacteria
作者: Lai, Hong-Zheng
Chen, Wei-Yu
Wu, Ching-Yi
Chen, Yu-Chie
應用化學系
Department of Applied Chemistry
關鍵字: pathogenic bacteria;antibiotics;gold nanoparticles;vancomycin;Staphylococcus aureus;macrophage
公開日期: 28-Jan-2015
摘要: Antibiotic-resistant bacteria have emerged because of the prevalent use of antibacterial agents. Thus, new antibacterial agents and therapeutics that can treat bacterial infections are necessary. Vancomycin is a potent antibiotic. Unfortunately, some bacterial strains have developed their resistance toward vancomycin. Nevertheless, it has been demonstrated that vancomycin-immobilized nanoparticles (NPs) are capable to be used in inhibition of the cell growth of vancomycin-resistant bacterial strains through multivalent interactions. However, multistep syntheses are usually necessary to generate vancomycin-immobilized NPs. Thus, maintaining the antibiotic activity of vancomycin when the drug is immobilized on the surface of NPs is challenging. In this study, a facile approach to generate vancomycin immobilized gold (Van-Au) NPs through one-pot stirring of vancomycin with aqueous tetrachloroauric acid at pH 12 and 25 degrees C for 24 h was demonstrated. Van-Au NPs (8.4 +/- 1.3 nm in size) were readily generated. The generated Van-Au NPs maintained their antibiotic activities and inhibited the cell growth of pathogens, which included Gram-positive and Gram-negative bacteria as well as antibiotic-resistant bacterial strains. Furthermore, the minimum inhibitory concentration of the Van-Au NPs against bacteria was lower than that of free-form vancomycin. Staphylococcus aureus-infected macrophages were used as the model samples to examine the antibacterial activity of the Van-Au NPs. Macrophages have the tendency to engulf Van-Au NPs through endocytosis. The results showed that the cell growth of S. aureus in the macrophages was effectively inhibited, suggesting the potential of using the generated Van-Au NPs as antibacterial agents for bacterial infectious diseases.
URI: http://dx.doi.org/10.1021/am507919m
http://hdl.handle.net/11536/124364
ISSN: 1944-8244
DOI: 10.1021/am507919m
期刊: ACS APPLIED MATERIALS & INTERFACES
起始頁: 2046
結束頁: 2054
Appears in Collections:Articles