標題: Inhibitory Effect of Excessive Glucose on Its Biochemical Pathway and the Growth of Chinese Hamster Ovary (CHO) Cells
作者: Lee, Tsung-Yih
Lin, Hsiao-Hsien
Chen, Chien-Lung
Hwang, Shiaw-Min
Tseng, Ching-Ping
生物科技學系
Department of Biological Science and Technology
關鍵字: Glucose and TCA cycle enzymes;Growth rate;ICDH;Glucose flux in glycolysis;CHO cells
公開日期: 2-一月-2015
摘要: In this study, we found the specific growth rate of CHO cells being reduced when glucose increased from 1 to 10g/L. Under high glucose, we show a 41% increase in lactate dehydrogenase activity consistent with a 70% rate increase in lactate production. The pyruvate kinase activity was significantly increased. However, the activities of citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase involved in the TCA cycle were attenuated. We propose that pyruvate produced from glycolysis is utilized in a lesser extent over the TCA cycle under high-glucose conditions. Thus, the pyruvate is largely converted to lactate, which is toxic to the cells.
URI: http://dx.doi.org/10.1080/07328303.2014.977908
http://hdl.handle.net/11536/124385
ISSN: 0732-8303
DOI: 10.1080/07328303.2014.977908
期刊: JOURNAL OF CARBOHYDRATE CHEMISTRY
Volume: 34
結束頁: 11
顯示於類別:期刊論文