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dc.contributor.authorSathyadevi, Palanisamyen_US
dc.contributor.authorChen, Yu-Jenen_US
dc.contributor.authorWu, Shou-Chengen_US
dc.contributor.authorChen, Yen-Haoen_US
dc.contributor.authorWang, Yun-Mingen_US
dc.date.accessioned2015-07-21T08:29:22Z-
dc.date.available2015-07-21T08:29:22Z-
dc.date.issued2015-06-15en_US
dc.identifier.issn0956-5663en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bios.2015.01.070en_US
dc.identifier.urihttp://hdl.handle.net/11536/124433-
dc.description.abstractHydrogen sulfide (H2S) has emerged as the most important biosynthetic gasotransmitters along with nitric oxide (NO) and carbon monoxide (CO). In this study, we report the design and the synthesis of a new epoxide fluorescent probe 7-glycidyloxy-9-(2-glycidyloxycarbonylphenyl)-2-xanthone (FEPO) for use in in vivo visualization of hydrogen sulfide. The probe employs a fluorescein as a fluorophore, and is equipped with an operating epoxide unit. FEPO functions via epoxide ring opening upon nucleophilic attack of H2S. This ring opening strategy may open a new avenue for the development of various H2S fluorescent sensors. FEPO showed high selectivity and high sensitivity for H2S. FEPO\'s cytotoxicity was tested using MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide) assay. Furthermore, the use of confocal imaging of H2S and in vivo imaging in live zebra fish demonstrated FEPO\'s potential biological applications. We anticipate that, owing to their ideal properties, probes of this type will find great uses in exploring the role of H2S in biology. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectFluoresceinen_US
dc.subjectH2S detectionen_US
dc.subjectConfocal imagingen_US
dc.subjectZebra fishen_US
dc.titleReaction-based epoxide fluorescent probe for in vivo visualization of hydrogen sulfideen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bios.2015.01.070en_US
dc.identifier.journalBIOSENSORS & BIOELECTRONICSen_US
dc.citation.volume68en_US
dc.citation.spage681en_US
dc.citation.epage687en_US
dc.contributor.department分子醫學與生物工程研究所zh_TW
dc.contributor.departmentInstitute of Molecular Medicine and Bioengineeringen_US
dc.identifier.wosnumberWOS:000351248300097en_US
dc.citation.woscount0en_US
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