標題: Label-free imaging of melanoma with nonlinear photothermal microscopy
作者: He, Jinping
Miyazaki, Jun
Wang, Nan
Tsurui, Hiromichi
Kobayashi, Takayoshi
電子物理學系
Department of Electrophysics
公開日期: 1-四月-2015
摘要: Nonlinear photothermal microscopy, in which the intensity of the pump heating beam is modulated at f and the photothermal signal is extracted from the probe beam with a lock-in amplifier referred to 2f, is applied to the imaging of mouse melanoma without any staining. The pump and probe pulses, with central wavelengths of 488 and 632 nm, and a pulse duration of similar to 100 ps, are filtered from a compact commercial supercontinuum fiber laser source. An auto-balanced detector is applied to accumulate the signal and remove the laser noise of the probe. The spatial resolution of the nonlinear photothermal imaging is enhanced by similar to 18% in both theoretical calculations and experiments, compared with a linear photothermal mechanism, and the resolution enhancement is theoretically similar to 42% compared with conventional optical microscopy. This imaging technique shows possibilities for the clinical evaluation of melanoma with a high contrast and spatial resolution. (C) 2015 Optical Society of America
URI: http://dx.doi.org/10.1364/OL.40.001141
http://hdl.handle.net/11536/124468
ISSN: 0146-9592
DOI: 10.1364/OL.40.001141
期刊: OPTICS LETTERS
Volume: 40
起始頁: 1141
結束頁: 1144
顯示於類別:期刊論文