標題: | Label-free imaging of melanoma with nonlinear photothermal microscopy |
作者: | He, Jinping Miyazaki, Jun Wang, Nan Tsurui, Hiromichi Kobayashi, Takayoshi 電子物理學系 Department of Electrophysics |
公開日期: | 1-四月-2015 |
摘要: | Nonlinear photothermal microscopy, in which the intensity of the pump heating beam is modulated at f and the photothermal signal is extracted from the probe beam with a lock-in amplifier referred to 2f, is applied to the imaging of mouse melanoma without any staining. The pump and probe pulses, with central wavelengths of 488 and 632 nm, and a pulse duration of similar to 100 ps, are filtered from a compact commercial supercontinuum fiber laser source. An auto-balanced detector is applied to accumulate the signal and remove the laser noise of the probe. The spatial resolution of the nonlinear photothermal imaging is enhanced by similar to 18% in both theoretical calculations and experiments, compared with a linear photothermal mechanism, and the resolution enhancement is theoretically similar to 42% compared with conventional optical microscopy. This imaging technique shows possibilities for the clinical evaluation of melanoma with a high contrast and spatial resolution. (C) 2015 Optical Society of America |
URI: | http://dx.doi.org/10.1364/OL.40.001141 http://hdl.handle.net/11536/124468 |
ISSN: | 0146-9592 |
DOI: | 10.1364/OL.40.001141 |
期刊: | OPTICS LETTERS |
Volume: | 40 |
起始頁: | 1141 |
結束頁: | 1144 |
顯示於類別: | 期刊論文 |