標題: Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility
作者: Hsieh, Yao-Yuan
Chang, Chi-Chen
Lin, Chich-Sheng
生物科技學系
Department of Biological Science and Technology
關鍵字: glutathione peroxidase;malondialdehyde;oligoasthenozoospermia;semen;sperm
公開日期: 1-Jan-2006
摘要: Objectives: Reactive oxygen species (ROS) induced lipid peroxidation is associated with sperm function. Malondialdehyde (MDA) concentration and glutathione peroxidase (GPx) activity represent the lipid peroxidation and spermicidal antioxidant, respectively. We aimed to evaluate the relationship of MDA and GPx levels with sperm parameters. Patients and methods: Specimens were divided into two groups: group 1. normospermia (n= 20); group 2. oligoasthenospermia (n= 31). Seminal MDA concentration was measured by thiobarbituric acid reaction method. Seminal GPx activity was measured by oxidation of reduced nicotinamide-adenine dinucleotide. Seminal MDA levels and GPx activities in both groups were compared. Results: MDA concentrations in both groups were significantly different (1.52 +/- 0.75 vs. 2.25 +/- 0.88 nM, p = 0.0021). GPx activities in both groups were non-significantly different (0.48 +/- 0.11 vs. 0.47 +/- 0.12 U/ml). MDA levels were negatively correlated with the sperm motility (MDA = -0.014 x motility + 2.62, p = 0.017) and concentration (MDA = -0.0045 x concentration + 2.23, p = 0.0166). GPx activities were positively but non-significantly correlated with the sperm concentration and sperm motility. Conclusions: Seminal MDA concentrations are negatively correlated with sperm concentration and motility, which might provide a simple and useful tool in predicting sperm parameters. GPx activity is non-significantly correlated with the seminal quality. Roles of seminal MDA upon spermatogenesis merits further surveys.
URI: http://hdl.handle.net/11536/124854
ISSN: 1449-2288
期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
起始頁: 23
結束頁: 29
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