利用常壓電漿改質聚乳酸蜂巢狀結構表面增強細胞生長效果之研究

Abstract

隨著人口不斷的上升以及醫療水準的進步，近年來組織工程變為極為熱門的研究領域，其中生醫材料則扮演當中重要的角色。而生醫材料的特性被歸納要包含無毒、具有生物相容性並且可以被生物降解等，而本實驗使用聚乳酸作為基底，正因為擁有上述這些性質，且被廣泛運用在生醫材料當中，搭配 breath figure method 來製備多孔薄膜結構，亦稱為蜂巢狀結構 (Honeycomb structure)，在許多的研究被認為適合細胞貼附及繁殖，實驗中可以製造出3-8 µm大小的孔洞。另外，研究室同仁所研發的兩階段常壓電漿改質設備，能有效將官能基接枝在材料表面上，而胺的官能基會使增進細胞貼附，經由電漿處理可以大幅度地提升表面的胺官能基。結合上述這兩種方法產生出的特性，來創造出細胞最有利的生長環境，並且調控電漿參數得到最佳的效果，來增加細胞的貼附和生長。將細胞培養在其表面上，使用多種的細胞搭配不同的孔洞大小的表面結構進行培養，結尾會探討不同種類細胞生長對於孔洞大小的關係，證實蜂巢狀結構表面進行電漿處理確實能有效增進細胞繁殖。

Recent years, with the improving of biomedical skill, biomaterial has been one of the popular issue in tissue engineering. Poly-lactictide (PLA) is non-toxic, biocompatible and degradable, which are essential characteristics for being biomaterial. Due to plenty advantages, PLA has been used widely. Honeycomb pattern (or breath figure pattern), a self-organized morphology, was firstly discovered in 1994. By creating this pattern on biomaterial surface may improve biocompatibility for enhancing cell attachment or proliferation. So far, our group has constructed a nitrogen-based planar DBD system. We mix nitrogen with few oxygen and ammonia, respectively. The device is applied to incorporate amine functional groups on flat PLA surface. The modified surface has been measured by XPS that the N/C ratio has raised. In this research, we expect to refine and combine these APPJ and honeycomb pattern together. We fabricate honeycomb pattern on PLA surface via breath figure method, and then use APPJ treatment to modify it. We focus on controlling pore sizes (3-8 µm) and optimizing conditions of APPJ treatment. Then to observe many kinds of cell culturing on the modified surface. We expect that the honeycomb-patterned surface could combine with the efficacy of APPJ surface modification, enhance the result of cell growth.