標題: | Graphene Oxide Based Nanocarrier Combined with a pH-Sensitive Tracer: A Vehicle for Concurrent pH Sensing and pH-Responsive Oligonucleotide Delivery |
作者: | Hsieh, Chia-Jung Chen, Yu-Cheng Hsieh, Pei-Ying Liu, Shi-Rong Wu, Shu-Pao Hsieh, You-Zung Hsu, Hsin-Yun 應用化學系 應用化學系分子科學碩博班 Department of Applied Chemistry Institute of Molecular science |
關鍵字: | graphene oxide;pH-responsive drug delivery;pH-sensitive rhodamine;oligonucleotide delivery;theranostics |
公開日期: | 3-六月-2015 |
摘要: | We chemically tuned the oxidation status of graphene oxide (GO) and constructed a GO-based nanoplatform combined with a pH-sensitive fluorescence tracer that is designed for both pH sensing and pH-responsive drug delivery. A series of GOs oxidized to distinct degrees were examined to optimize the adsorption of the model drug, poly dT(30). We determined that highly oxidized GO was a superior drug-carrier candidate in vitro when compared to GOs oxidized to lesser degrees. In the cell experiment, the synthesized pH-sensitive rhodamine dye was first applied to monitor cellular pH; under acidic conditions, protonated rhodamine fluoresces at 588 mm (lambda(ex) = 561 nm). When the nanocarrier was introduced into cells, a rhodamine-triggered competition reaction occurred, and this led to the release of the oligonucleotides and the quenching of rhodamine fluorescence by GO. Our results indicate high drug loading (FAM-dT(30)/GO = 25/50 mu g/mL) and rapid cellular uptake (<0.5 h) of the nanocarrier which can potentially be used for targeted RNAi delivery to the acidic milieu of tumors. |
URI: | http://dx.doi.org/10.1021/acsami.5b02397 http://hdl.handle.net/11536/127896 |
ISSN: | 1944-8244 |
DOI: | 10.1021/acsami.5b02397 |
期刊: | ACS APPLIED MATERIALS & INTERFACES |
Issue: | 21 |
起始頁: | 11467 |
結束頁: | 11475 |
顯示於類別: | 期刊論文 |