標題: Graphene Oxide Based Nanocarrier Combined with a pH-Sensitive Tracer: A Vehicle for Concurrent pH Sensing and pH-Responsive Oligonucleotide Delivery
作者: Hsieh, Chia-Jung
Chen, Yu-Cheng
Hsieh, Pei-Ying
Liu, Shi-Rong
Wu, Shu-Pao
Hsieh, You-Zung
Hsu, Hsin-Yun
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: graphene oxide;pH-responsive drug delivery;pH-sensitive rhodamine;oligonucleotide delivery;theranostics
公開日期: 3-六月-2015
摘要: We chemically tuned the oxidation status of graphene oxide (GO) and constructed a GO-based nanoplatform combined with a pH-sensitive fluorescence tracer that is designed for both pH sensing and pH-responsive drug delivery. A series of GOs oxidized to distinct degrees were examined to optimize the adsorption of the model drug, poly dT(30). We determined that highly oxidized GO was a superior drug-carrier candidate in vitro when compared to GOs oxidized to lesser degrees. In the cell experiment, the synthesized pH-sensitive rhodamine dye was first applied to monitor cellular pH; under acidic conditions, protonated rhodamine fluoresces at 588 mm (lambda(ex) = 561 nm). When the nanocarrier was introduced into cells, a rhodamine-triggered competition reaction occurred, and this led to the release of the oligonucleotides and the quenching of rhodamine fluorescence by GO. Our results indicate high drug loading (FAM-dT(30)/GO = 25/50 mu g/mL) and rapid cellular uptake (<0.5 h) of the nanocarrier which can potentially be used for targeted RNAi delivery to the acidic milieu of tumors.
URI: http://dx.doi.org/10.1021/acsami.5b02397
http://hdl.handle.net/11536/127896
ISSN: 1944-8244
DOI: 10.1021/acsami.5b02397
期刊: ACS APPLIED MATERIALS & INTERFACES
Issue: 21
起始頁: 11467
結束頁: 11475
顯示於類別:期刊論文