標題: | Highly sensitive color-indicating and quantitative biosensor based on cholesteric liquid crystal |
作者: | Hsiao, Yu-Cheng Sung, Yu-Chien Lee, Mon-Juan Lee, Wei 影像與生醫光電研究所 Institute of Imaging and Biomedical Photonics |
公開日期: | 1-十二月-2015 |
摘要: | Liquid crystal (LC)-based biosensors employ highly sensitive interfaces between the alignment layers and LCs to detect biomolecules and their interactions. Present techniques based on optical texture observation of the homeotropic-to-planar response of nematic LCs are limited by their quantitative reproducibility of results, indicating that both the accuracy and reliability of LC-based detection require further improvements. Here we show that cholesteric LC (CLC) can be used as a novel sensing element in the design of an alternative LC-based biosensing device. The chirality of the vertically anchored (VA) CLC was exploited in the detection of bovine serum albumin (BSA), a protein standard commonly used in protein quantitation. The color appearance and the corresponding transmission spectrum of the cholesteric phase changed with the concentration of BSA, by which a detection limit of 1 fg/ml was observed. The optical response of the VA CLC interface offers a simple and inexpensive platform for highly sensitive and naked-eye color-indicating detection of biomolecules, and, thus, may facilitate the development of point-of-care devices for the detection of disease-related biomarkers. (C) 2015 Optical Society of America |
URI: | http://dx.doi.org/10.1364/BOE.6.005033 http://hdl.handle.net/11536/129341 |
ISSN: | 2156-7085 |
DOI: | 10.1364/BOE.6.005033 |
期刊: | BIOMEDICAL OPTICS EXPRESS |
Volume: | 6 |
Issue: | 12 |
起始頁: | 5033 |
結束頁: | 5038 |
顯示於類別: | 期刊論文 |