標題: In vitro investigation of methylene blue-bearing, electrostatically assembled aptamer-silica nanocomposites as potential photodynamic therapeutics
作者: Ding, Tian-Shyng
Huang, Xin-Chun
Luo, Yun-Ling
Hsu, Hsin-Yun
應用化學系分子科學碩博班
Institute of Molecular science
關鍵字: Aptamer-silica nanocomposite;Methylene blue;Electrostatic assembly;Singlet oxygen;Photodynamic therapy
公開日期: 1-Nov-2015
摘要: Photodynamic therapy, that is, excitation of a photosensitizer with light to generate reactive oxygen species such as singlet oxygen, has emerged as a noninvasive technique for cancer theranostics. However, the clinical use of many photosensitizers is impeded by their hydrophobicity, the nonspecific damage they cause to normal tissues, and their susceptibility to environmental degradation. In this study, we developed a simple electrostatic adsorption strategy to fabricate aptamer silica nanocomposites by sequentially functionalizing nanocomposites with the cell surface-associated mucin 1 aptamer for tumor targeting and a hydrophilic photosensitizer, methylene blue, for photodynamic therapy applications. We investigated the relationship between the biophysical properties and cellular uptake of such nanocomposites to improve their formulation. Effective generation of singlet oxygen was achieved with a low photosensitizer dosage (0.5 mu M) and a short, low-power irradiation (1 min, 10 mW/cm(2)). With the current strategy, the efficiency of photodynamic therapy was determined by the cellular uptake of nanocomposites and the targeting molecules used. (C) 2015 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.colsurfb.2015.07.064
http://hdl.handle.net/11536/129578
ISSN: 0927-7765
DOI: 10.1016/j.colsurfb.2015.07.064
期刊: COLLOIDS AND SURFACES B-BIOINTERFACES
Volume: 135
起始頁: 217
結束頁: 224
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