標題: | In Situ Activation of Pituitary-Infiltrating T Lymphocytes in Autoimmune Hypophysitis |
作者: | Lin, Han-Huei Gutenberg, Angelika Chen, Tzu-Yu Tsai, Nu-Man Lee, Chia-Jung Cheng, Yu-Che Cheng, Wen-Hui Tzou, Ywh-Min Caturegli, Patrizio Tzou, Shey-Cherng 生物科技學系 分子醫學與生物工程研究所 Department of Biological Science and Technology Institute of Molecular Medicine and Bioengineering |
公開日期: | 6-Mar-2017 |
摘要: | Autoimmune hypophysitis (AH) is a chronic inflammatory disease characterized by infiltration of T and B lymphocytes in the pituitary gland. The mechanisms through which infiltrating lymphocytes cause disease remain unknown. Using a mouse model of AH we assessed whether T lymphocytes undergo activation in the pituitary gland. Infiltrating T cells co-localized with dendritic cells in the pituitary and produced increased levels of interferon-gamma and interleukin-17 upon stimulation in vitro. Assessing proliferation of CD3- and B220-postive lymphocytes by double immunohistochemistry (PCNA-staining) and flow cytometry (BrdU incorporation) revealed that a discrete proportion of infiltrating T cells and B cells underwent proliferation within the pituitary parenchyma. This proliferation persisted into the late disease stage (day 56 post-immunization), indicating the presence of a continuous generation of autoreactive T and B cells within the pituitary gland. T cell proliferation in the pituitary was confirmed in patients affected by autoimmune hypophysitis. In conclusion, we show that pituitary-infiltrating lymphocytes proliferate in situ during AH, providing a previously unknown pathogenic mechanism and new avenues for treatment. |
URI: | http://dx.doi.org/10.1038/srep43492 http://hdl.handle.net/11536/133138 |
ISSN: | 2045-2322 |
DOI: | 10.1038/srep43492 |
期刊: | SCIENTIFIC REPORTS |
Volume: | 7 |
起始頁: | 0 |
結束頁: | 0 |
Appears in Collections: | Articles |
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