標題: Sodium Dodecyl Sulfate-Modified Doxorubicin-Loaded Chitosan-Lipid Nanocarrier with Multi Polysaccharide-Lecithin Nanoarchitecture for Augmented Bioavailability and Stability of Oral Administration In Vitro and In Vivo
作者: Su, Chia-Wei
Chiang, Min-Yu
Lin, Yu-Ling
Tsai, Nu-Man
Chen, Yen-Po
Li, Wei-Ming
Hsu, Chin-Hao
Chen, San-Yuan
材料科學與工程學系
分子醫學與生物工程研究所
Department of Materials Science and Engineering
Institute of Molecular Medicine and Bioengineering
關鍵字: Oral Delivery;Triglyceride;Caco-2 Cell Monolayers;Intestinal Absorption;Bioavailability
公開日期: May-2016
摘要: For oral anti-cancer drug delivery, a new chitosan-lipid nanoparticle with sodium dodecyl sulfate modification was designed and synthesized using a double emulsification. TEM examination showed that the DOX-loaded nanoparticles, termed D-PL/TG NPs, exhibited a unique core shell configuration composed of multiple amphiphilic chitosan-lecithin reverse micelles as the core and a triglyceride shell as a physical barrier to improve the encapsulation efficiency and reduce the drug leakage. In addition, the D-PL/TG NPs with sodium dodecyl sulfate modification on the surface have enhanced stability in the GI tract and increased oral bioavailability of doxorubicin. In vitro transport studies performed on Caco-2 monolayers indicated that the D-PL/TG NPs enhanced the permeability of DOX in the Caco-2 monolayers by altering the transport pathway from passive diffusion to transcytosis. The in vivo intestinal absorption assay suggested that the D-PL/TG NPs were preferentially absorbed through the specialized membranous epithelial cells (M cells) of the Peyer\'s patches, resulting in a significant improvement (8-fold) in oral bioavailability compared to that of free DOX. The experimental outcomes in this work demonstrate that the D-PL/TG NPs provide an exciting opportunity for advances in the oral administration of drugs with poor bioavailability that are usually used in treating tough and chronic diseases.
URI: http://dx.doi.org/10.1166/jbn.2016.2227
http://hdl.handle.net/11536/133642
ISSN: 1550-7033
DOI: 10.1166/jbn.2016.2227
期刊: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume: 12
Issue: 5
起始頁: 962
結束頁: 972
Appears in Collections:Articles