標題: Nucleotide-Dependent Bioautocatalytic Timer Reaction
作者: Chen, Ting-Ru
Hsu, Ching-Fong
Chen, Chih-Lin
Witek, Henryk A.
Urban, Pawel L.
應用化學系
Department of Applied Chemistry
關鍵字: amplification;luminescence;nonlinear chemistry;nucleotide;time
公開日期: Sep-2016
摘要: We describe a biochemical timer composed of three biocatalytic reactions involving three types of adenylate nucleotides: adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP). The timer is triggered by a small amount of ATP or ADP. An abrupt increase in the ATP concentration (following numerous amplification cycles) leads to a sudden increase of luminescence from the reaction mixture. The time point when the luminescence appears is found to be a function of the initial concentration of the triggering nucleotide (5.0 X 10(-8)-1.0 X 10(-6) M), even in the presence of a complex biological matrix. The mechanism of the observed dependence of the time of luminescence increase on the concentration has been confirmed with simple kinetic models. Due to the biocompatibility of the proposed trienzymatic reaction scheme (sensitivity to common nucleotides and occurrence in a neutral pH aqueous environment), the scheme can be used in bioengineered systems that require modulation of the response time (light emission) by concentration.
URI: http://dx.doi.org/10.1021/acssynbio.6b00116
http://hdl.handle.net/11536/134253
ISSN: 2161-5063
DOI: 10.1021/acssynbio.6b00116
期刊: ACS SYNTHETIC BIOLOGY
Volume: 5
Issue: 9
起始頁: 962
結束頁: 968
Appears in Collections:Articles