標題: CAPS Genomic Subtyping on Orthomyxoviridae
作者: Peng, Sheng-Lung
Tsay, Yu-Wei
Lin, Chich-Sheng
Tang, Chuan Yi
生物科技學系
Department of Biological Science and Technology
公開日期: 2009
摘要: The Orthomyxoviridae is a family of single strained RNA viruses including five genera: Influenza virus A, Influenza virus B, Influenza virus C, Thogotovirus, and Isavirus. Usually, Influenza viruses are identified by antigenic differences in their nucleoprotein and matrix protein. In this paper, we propose an algorithm to determine a set of suitable: restriction enzymes for producing recognizable restriction maps on Orthomyxoviridae. Our method is applied to viral strains of highly pathogenic avian influenza (HPAI), containing potentially homozygous, heterozygous; and various genetic variations. In the analysis of CAPS (Cleaved Amplified Polymorphic Sequence) subtyping, our method out; performs the RNA coding of representative and epidemiologically significant human wild-type viruses, including H3N8, H5N1, H5N9, H7N1, H7N7, and H9N2. These isolates are analyzed by CAPS with enzymes Agel, EciI, KpnI, and XbaI. The HPAI strains show a. different RFLP (Restriction Fragment Length Polymorphism) profile by comparing with other low pathogenic avian influenza (LPAI) strains. We provide a rapid; specific, and reproducible identification of the genotypes on Orthomyxoviridae It permits us to quickly confirm subtypes of Orthomyxoviridae.
URI: http://hdl.handle.net/11536/136518
ISBN: 978-3-642-04069-6
ISSN: 0302-9743
期刊: EMERGING INTELLIGENT COMPUTING TECHNOLOGY AND APPLICATIONS, PROCEEDINGS
Volume: 5754
起始頁: 130
結束頁: +
Appears in Collections:Conferences Paper