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dc.contributor.authorWu, YTen_US
dc.contributor.authorChen, YCen_US
dc.date.accessioned2014-12-08T15:19:22Z-
dc.date.available2014-12-08T15:19:22Z-
dc.date.issued2005-04-01en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://dx.doi.org/10.1021/ac048349ien_US
dc.identifier.urihttp://hdl.handle.net/11536/13828-
dc.description.abstractIt has always been assumed that electrical contact at the capillary outlet is a necessary requirement when coupling capillary electropboresis (CE) with electrospray ionization mass spectrometry (ESI-MS). In this study, we used a pulled bare-capillary tip as the ESI emitter, but neither was it coated with any electrically conductive materials nor was a high external voltage applied on its outlet. In this paper, we demonstrate that this straightforward approach may be used to generate multiply charged ions of proteins and peptides through electrospray ionization. Our results indicate that peptides and proteins, including bradykinin, cytochrome c, myoglobin, and tryptic digest products that elute from a pulled bare-capillary tip can be detected directly by ESI-MS using the tapered bare-capillary interface. Thus, we have demonstrated that CE and ESI-MS may be combined successfully without the need to modify the outlet of the capillary tip with an electrically contacting material.en_US
dc.language.isoen_USen_US
dc.titleSheathless capillary electrophoresis/electrospray ionization mass spectrometry using a pulled bare fused-silica capillary as the electrospray emitteren_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac048349ien_US
dc.identifier.journalANALYTICAL CHEMISTRYen_US
dc.citation.volume77en_US
dc.citation.issue7en_US
dc.citation.spage2071en_US
dc.citation.epage2077en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000228231400027-
dc.citation.woscount19-
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