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dc.contributor.authorLin, YSen_US
dc.contributor.authorTsai, PJen_US
dc.contributor.authorWeng, MFen_US
dc.contributor.authorChen, YCen_US
dc.date.accessioned2014-12-08T15:19:31Z-
dc.date.available2014-12-08T15:19:31Z-
dc.date.issued2005-03-15en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://dx.doi.org/10.1021/ac048990ken_US
dc.identifier.urihttp://hdl.handle.net/11536/13898-
dc.description.abstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides a straightforward means to differentiate microorganism species based on mass spectral fingerprinting. The pathogen cell concentration in an infected sample, however, is generally lower than that capable of being detected directly by MALDI-MS. Furthermore, the presence of proteins or metabolites in biological fluids always causes unavoidable interference for the identification of microorganism species. Vancomycin, which binds to D-Ala-D-Ala moieties on the cell walls of Gram-positive bacteria and, therefore, inhibits peptidoglycan synthesis, is one of the most potent antibiotics. Thus, we have employed vancomycin-modified magnetic nanoparticles as affinity probes to selectively trap Gram-positive pathogens from sample solutions; i.e., these bacteria can be isolated from sample solutions by applying a magnetic field. The isolated cells could then be characterized by MALDI-MS. This approach effectively reduces the interference of protein and metabolite signals in the mass spectra of Gram-positive bacteria because vancomycin has such high specificity for the D-Ala-D-Ala units of the cell walls. The lowest cell concentration we detected for both Staphylococcus saprophyticus and Staphylococcus aureus in a urine sample (3 mL) was similar to 7 x 10(4) cfu/mL.en_US
dc.language.isoen_USen_US
dc.titleAffinity capture using vancomycin-bound magnetic nanoparticles for the MALDI-MS analysis of bacteriaen_US
dc.typeArticleen_US
dc.identifier.doi10.1021/ac048990ken_US
dc.identifier.journalANALYTICAL CHEMISTRYen_US
dc.citation.volume77en_US
dc.citation.issue6en_US
dc.citation.spage1753en_US
dc.citation.epage1760en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000227759500028-
dc.citation.woscount123-
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