標題: Using biofunctionalized nanoparticles to probe pathogenic bacteria
作者: Ho, KC
Tsai, PJ
Lin, YS
Chen, YC
應用化學系
Department of Applied Chemistry
公開日期: 15-十二月-2004
摘要: In this paper, we report a method for fabricating biofunctionalized nanoparticles by attaching human immunoglobulin (IgG) onto their surfaces through either electrostatic interactions or covalent binding. We found that these IgG-presenting nanoparticles can bind selectively to the cell walls of pathogens that contain IgG-binding sites based on the investigation of transmission electron microscopy images. Our results demonstrate that such Au-IgG nanoparticles may serve as useful nanoscale probes for exploring the interactions between IgG and pathogens. Furthermore, the IgG-presenting magnetic nanoparticles have been employed as effective affinity probes for selectively concentrating traces of target bacteria from sample solutions. The trapped bacteria were then characterized by using matrix-assisted laser desorption/ionization mass spectrometry. The lowest cell concentration we detected for both Staphylococcus saprophyticus and Staphylococcus aureus in aqueous sample solutions (0.5 mL) was similar to3 x 10(5) cfu/mL, while the detectable cell concentration for S. saprophyticus in a urine sample was similar to3 x 10(7) cfu/mL.
URI: http://dx.doi.org/10.1021/ac048688b
http://hdl.handle.net/11536/25549
ISSN: 0003-2700
DOI: 10.1021/ac048688b
期刊: ANALYTICAL CHEMISTRY
Volume: 76
Issue: 24
起始頁: 7162
結束頁: 7168
顯示於類別:期刊論文


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