以藍相液晶作為牛血清蛋白之生物檢測平台

Abstract

本論文為液晶學術領域中，首次運用藍相態作為生醫檢測應用之研究；利用藍相液晶之布拉格反射，以光譜分析來界定受測之牛血清蛋白質含量，突破液晶生醫檢測難以量化的問題。 實驗所採用的藍相液晶材料有兩種，分別有著不同的檢測效果，在以E7參雜R5011的材料之中，配合DMOAP的配向，生成均勻的藍相態並造成了強烈的布拉格反射，而牛血清蛋白質的存在會影響藍相的晶面一致性，在結果中得到，蛋白質濃度高於106 g/mL時，光譜可偵測到兩個峰值，而低於106 g/mL則會隨著牛血清蛋白的濃度改變其反射峰強度；而在E7參雜S811的材料結果中，牛血清蛋白質濃度低於106 g/mL，藍相光譜的訊號峰會隨著牛血清蛋白濃度之提高而藍移，但在牛血清蛋白濃度高於106 g/mL時，藍相結構會太過於混亂，造成大量散射無法分析反射峰，而研究中利用了此材料的層列向態輔佐，在牛血清蛋白濃度高於106 g/mL的情形下，可利用層列向之穿透光譜作為分析牛血清蛋白濃度的手段。

In this study, it is the first time that blue phase liquid crystals are used for biomedical detection. Because of the Bragg reflection caused by the blue phase liquid crystals, we can observe the change of the blue phase reflection peak from different BSA concentration sample and realize the quantification in liquid crystal biomedical detection. In the experiment, there are two different mixture being investigated and the effects of detection from each mixture are also different. In the first part result of the E7 doped with chiral R5011, the uniform blue phase structure with DMOAP alignment showed sharp Bragg reflection peak and the presence of BSA disturbed the alignment of blue phase liquid crystal, causing the multi-domain lattice structure. In the result, two reflection peak appeared in the spectrum while the BSA concentration was higher than 106 g/mL, or there was only one peak on spectrum and the intensity of the only peak increased as the BSA concentration decreased. The other result was from mixture of E7 doped with S811 and the Bragg reflection peak blue shifted as the BSA concentration increased only if the concentration was lower than 106 g/mL. When the BSA concentration was higher than 106 g/mL, the strong scattering happened induced by the disorder lattice. Thus we used the spectrum of smectic A phase which was appeared at lower temperature in the same mixture to assist in detecting the BSA concentration while the concentration is higher than 106 g/mL.