標題: | Detection of chlorophylls in spores of seven ferns |
作者: | Tseng, Mei-Hwei Lin, Kuei-Huei Huang, Yi-Jia Chang, Ya-Lan Huang, Sheng-Cih Kuo, Li-Yaung Huang, Yao-Moan 應用化學系 Department of Applied Chemistry |
關鍵字: | Chlorophyll;Cryptochlorophyllous spore;Spore-squash epifluorescence;UHPLC-UV-MS |
公開日期: | 1-三月-2017 |
摘要: | Fern spores were traditionally classified into chlorophyllous (green) and nonchlorophyllous (nongreen) types based on the color visible to the naked eye. Recently, a third type, "cryptochlorophyllous spores", is recognized, and these spores are nongreen under white light but contain chlorophylls. Epifluorescence microscopy was previously used to detect chlorophylls in cryptochlorophyllous spores. In addition to epifluorescence microscopy, current study performed some other approaches, including spore-squash epifluorescence, absorption spectra, laser-induced fluorescence emission spectra, thin layer chromatography (TLC), and ultra-high performance liquid chromatography with ultraviolet and mass spectrometric detection (UHPLC-UV-MS) in order to detect chlorophylls of spores of seven ferns (Sphaeropteris lepifera, Ceratopteris thalictroides, Leptochilus wrightii, Leptochilus pothifolius, Lepidomicrosorum buergerianum, Osmunda banksiifolia, and Platycerium grande). Destructive methods, such as TLC and UHPLC-UV-MS, successfully detected chlorophylls inside the spores when their signals of red fluorescence under epifluorescence microscope were masked by spore wall. Although UHPLC-UV-MS analysis was the most sensitive and reliable for determining the chlorophylls of spores, spore-squash epifluorescence is not only reliable but also cost- and time-effective one among our study methods. In addition, we first confirmed that Lepidomicrosorium buergerianum, Leptochilus pothifolius, Leptochilus wrightii, and Platycerium grande, produce cryptochlorophyllous spores. |
URI: | http://dx.doi.org/10.1007/s10265-016-0901-5 http://hdl.handle.net/11536/143965 |
ISSN: | 0918-9440 |
DOI: | 10.1007/s10265-016-0901-5 |
期刊: | JOURNAL OF PLANT RESEARCH |
Volume: | 130 |
起始頁: | 407 |
結束頁: | 416 |
顯示於類別: | 期刊論文 |