標題: Comparative microarray analyses of mono(2-ethylhexyl)phthalate impacts on fat cell bioenergetics and adipokine network
作者: Chiang, Huai-Chih
Wang, Chih-Hong
Yeh, Szu-Ching
Lin, Yi-Hua
Kuo, Ya-Ting
Liao, Chih-Wei
Tsai, Feng-Yuan
Lin, Wei-Yu
Chuang, Wen-Han
Tsou, Tsui-Chun
生物科技學系
Department of Biological Science and Technology
關鍵字: Phthalates;Endocrine disruptor;Energy metabolism;PPAR gamma;Adipocytes
公開日期: 1-Dec-2017
摘要: Cellular accumulation of mono(2-ethylhexyl)phthalate (MEHP) has been recently demonstrated to disturb fat cell energy metabolism; however, the underlying mechanism remained unclear. The study aimed to determine how MEHP influenced fat cell transcriptome and how the changes might contribute to bioenergetics. Because of the pivotal role of PPAR gamma in energy metabolism of fat cells, comparative microarray analysis of gene expression in 3T3-L1 adipocytes treated with both MEHP and rosiglitazone was performed. Pathway enrichment analysis and gene ontology (GO) enrichment analysis revealed that both treatments caused up-regulation of genes involved in PPAR signaling/energy metabolism-related pathways and down-regulation of genes related to adipokine/inflammation signals. MEHP/rosiglitazone-treated adipocytes exhibited increased levels of lipolysis, glucose uptake, and glycolysis; the gene expression profiles provided molecular basis for the functional changes. Moreover, MEHP was shown to induce nuclear translocation and activation of PPAR gamma. The similarity in gene expression and functional changes in response to MEHP and rosiglitazone suggested that MEHP influenced bioenergetics and adipokine network mainly via PPAR gamma. Importantly, adipokine levels in C57BL/6J mice with di(2-ethylhexyl)phthalate (DEHP) treatments provided in vivo evidence for microarray results. On the basis of correlation between gene expression and functional assays, possible involvements of genes in bioenergetics of MEHP-treated adipocytes were proposed.
URI: http://dx.doi.org/10.1007/s10565-016-9380-7
http://hdl.handle.net/11536/144004
ISSN: 0742-2091
DOI: 10.1007/s10565-016-9380-7
期刊: CELL BIOLOGY AND TOXICOLOGY
Volume: 33
起始頁: 511
結束頁: 526
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