標題: Nanodiamonds conjugated to gold nanoparticles for colorimetric detection of clenbuterol and chromium(III) in urine
作者: Shellaiah, Muthaiah
Simon, Turibius
Venkatesan, Parthiban
Sun, Kien Wen
Ko, Fu-Hsiang
Wu, Shu-Pao
材料科學與工程學系
應用化學系
電子工程學系及電子研究所
Department of Materials Science and Engineering
Department of Applied Chemistry
Department of Electronics Engineering and Institute of Electronics
關鍵字: Gold nanoparticles;Nanocomposite;beta-Adrenergic drug assay;Chromium(III) recognition;pH effective sensor;Surface plasmon resonance;Sub-nanomolar detection;Urine analysis;Water analysis
公開日期: 1-Jan-2018
摘要: Nanodiamonds were modified such that they carry thiol groups (ND-thiol). Gold nanoparticles were reacted with ND-thiol to obtain a highly stable conjugate of the type ND@AuNPs. Both ND-thiol and the ND@AuNPs were characterized by SEM, TEM, AFM, DLS, zeta potential, XPS, XRD, UV-Vis, Raman, FTIR and cytotoxicity studies. Their biocompatibility was confirmed via an MTT assay with HeLa cells. At a pH value of 6, the ND@AuNPs represent a colorimetric probe that can be used to selectively detect the illegally used beta-adrenergic drug clenbuterol (CLB) and the pollutant chromium(III). Detection can be performed visually by monitoring the color change from wine red to purple blue, or by colorimetric measurement of the so-called SPR peaks at 651 and 710 nm. The color changes are due to aggregation, and this is confirmed by TEM and DLS data. The involvement of surface functional groups that assist in analyte recognition was verified by FTIR. The detection limits are 0.49 nM for CLB, and 0.37 nM for Cr(III). The ND@AuNPs were successfully applied to the determination of Cr(III) and CLB in spiked human urine samples. Notably, the low interference by other ions in the detection of Cr(III) in tap and lake water is confirmed by ICP-MS analyses.
URI: http://dx.doi.org/10.1007/s00604-017-2611-7
http://hdl.handle.net/11536/144371
ISSN: 0026-3672
DOI: 10.1007/s00604-017-2611-7
期刊: MICROCHIMICA ACTA
Volume: 185
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