標題: LED-CT Scan for pH Distribution on a Cross-Section of Cell Culture Medium
作者: Higashino, Nobuya
Takayama, Toshio
Ito, Hiroaki
Horade, Mitsuhiro
Yamaguchi, Yasutaka
Tsai, Chia-Hung Dylan
Kaneko, Makoto
機械工程學系
Department of Mechanical Engineering
關鍵字: pH measurement;cell culture;non-contact measurement
公開日期: 1-Jan-2018
摘要: In cell culture, the pH of the culture medium is one of the most important conditions. However, the culture medium may have non-uniform pH distribution due to activities of cells and changes in the environment. Although it is possible to measure the pH distribution with an existing pH meter using distributed electrodes, the method involves direct contact with the medium and would greatly increase the risk of contamination. Here in this paper, we propose a computed tomography (CT) scan for measuring pH distribution using the color change of phenol red with a light-emitting diode (LED) light source. Using the principle of CT scan, we can measure pH distribution without contacting culture medium, and thus, decrease the risk of contamination. We have developed the device with a LED, an array of photo receivers and a rotation mechanism. The system is firstly calibrated with different shapes of wooden objects that do not pass light, we succeeded in obtaining their 3D topographies. The system was also used for measuring a culture medium with two different pH values, it was possible to obtain a pH distribution that clearly shows the boundary.
URI: http://dx.doi.org/10.3390/s18010191
http://hdl.handle.net/11536/144428
ISSN: 1424-8220
DOI: 10.3390/s18010191
期刊: SENSORS
Volume: 18
Appears in Collections:Articles