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dc.contributor.authorChang, Chia-Yuanen_US
dc.contributor.authorLin, Cheng-Hanen_US
dc.contributor.authorLin, Chun-Yuen_US
dc.contributor.authorSie, Yong-Daen_US
dc.contributor.authorHu, Yvonne Yulingen_US
dc.contributor.authorTsai, Sheng-Fengen_US
dc.contributor.authorChen, Shean-Jenen_US
dc.date.accessioned2018-08-21T05:53:19Z-
dc.date.available2018-08-21T05:53:19Z-
dc.date.issued2018-01-01en_US
dc.identifier.issn1864-063Xen_US
dc.identifier.urihttp://dx.doi.org/10.1002/jbio.201600287en_US
dc.identifier.urihttp://hdl.handle.net/11536/144539-
dc.description.abstractA developed temporal focusing-based multiphoton excitation microscope (TFMPEM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPEM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPEM can be condensed from 3.0 mm to 1.5 mm for a 50% improvement. By using the TFMPEM with HiLo technique as two structured illuminations at the same spatial frequency but different orientation, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression. Picture: TPEF images of the eosin-stained mouse cerebellar cortex by conventional TFMPEM (left), and the TFMPEM with HiLo technique as 1.09 mm(-1) spatially modulated illumination at 908 (center) and 08 (right) orientations. [GRAPHICS] .en_US
dc.language.isoen_USen_US
dc.subjectmultiphoton processesen_US
dc.subjectnonlinear microscopyen_US
dc.subjectthree-dimensional microscopyen_US
dc.titleTemporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imagingen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/jbio.201600287en_US
dc.identifier.journalJOURNAL OF BIOPHOTONICSen_US
dc.citation.volume11en_US
dc.contributor.department光電工程學系zh_TW
dc.contributor.departmentDepartment of Photonicsen_US
dc.identifier.wosnumberWOS:000425294600013en_US
Appears in Collections:Articles