標題: A Three-MicroRNA Signature as a Potential Biomarker for the Early Detection of Oral Cancer
作者: Chang, Yi-An
Weng, Shun-Long
Yang, Shun-Fa
Chou, Chih-Hung
Huang, Wei-Chih
Tu, Siang-Jyun
Chang, Tzu-Hao
Huang, Chien-Ning
Jong, Yuh-Jyh
Huang, Hsien-Da
生物科技學系
生物資訊及系統生物研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
關鍵字: miRNA;biomarker;oral cancer;leukoplakia;early diagnosis
公開日期: 1-Mar-2018
摘要: Oral squamous cell carcinoma (OSCC) is often diagnosed at a late stage and may be malignantly transformed from oral leukoplakia (OL). This study aimed to identify potential plasma microRNAs (miRNAs) for the early detection of oral cancer. Plasma from normal, OL, and OSCC patients were evaluated. Small RNA sequencing was used to screen the differently expressed miRNAs among the groups. Next, these miRNAs were validated with individual samples by quantitative real-time polymerase chain reaction (qRT-PCR) assays in the training phase (n = 72) and validation phase (n = 178). The possible physiological roles of the identified miRNAs were further investigated using bioinformatics analysis. Three miRNAs (miR-222-3p, miR-150-5p, and miR-423-5p) were identified as differentially expressed among groups; miR-222-3p and miR-423-5p negatively correlated with T stage, lymph node metastasis status, and clinical stage. A high diagnostic accuracy (Area under curve = 0.88) was demonstrated for discriminating OL from OSCC. Bioinformatics analysis reveals that miR-423-5p and miR-222-3p are significantly over-expressed in oral cancer tissues and involved in various cancer pathways. The three-plasma miRNA panel may be useful to monitor malignant progression from OL to OSCC and as potential biomarkers for early detection of oral cancer.
URI: http://dx.doi.org/10.3390/ijms19030758
http://hdl.handle.net/11536/144727
ISSN: 1422-0067
DOI: 10.3390/ijms19030758
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 19
Appears in Collections:Articles