標題: Rapid localized crystallization of lysozyme by laser trapping
作者: Yuyama, Ken-ichi
Chang, Kai-Di
Tu, Jing-Ru
Masuhara, Hiroshi
Sugiyama, Teruki
應用化學系
Department of Applied Chemistry
公開日期: 7-Mar-2018
摘要: Confining protein crystallization to a millimetre size was achieved within 0.5 h after stopping 1 h intense trapping laser irradiation, which shows excellent performance in spatial and temporal controllability compared to spontaneous nucleation. A continuous-wave near-infrared laser beam is tightly focused into a glass/solution interfacial layer of a supersaturated buffer solution of hen egg-white lysozyme (HEWL). The crystallization is not observed during laser trapping, but initiated by stopping the laser irradiation. The generated crystals are localized densely in a circular area with a diameter of a few millimetres around the focal spot and show specific directions of the optical axes of the HEWL crystals. To interpret this unique crystallization, we propose a mechanism that nucleation and the subsequent growth take place in a highly concentrated domain consisting of HEWL liquid-like clusters after turning off laser trapping.
URI: http://dx.doi.org/10.1039/c7cp06990a
http://hdl.handle.net/11536/144802
ISSN: 1463-9076
DOI: 10.1039/c7cp06990a
期刊: PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume: 20
起始頁: 6034
結束頁: 6039
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