標題: DNA sequencing using electrical conductance measurements of a DNA polymerase (Retracted article. See vol. 10, pg. 563, 2015)
作者: Chen, Yu-Shiun
Lee, Chia-Hui
Hung, Meng-Yen
Pan, Hsu-An
Chiou, Jin-Chern
Huang, G. Steven
材料科學與工程學系
生物科技學系
電控工程研究所
生醫電子轉譯研究中心
Department of Materials Science and Engineering
Department of Biological Science and Technology
Institute of Electrical and Control Engineering
Biomedical Electronics Translational Research Center
公開日期: 1-Jun-2013
摘要: The development of personalized medicine-in which medical treatment is customized to an individual on the basis of genetic information-requires techniques that can sequence DNA quickly and cheaply. Single-molecule sequencing technologies, such as nanopores, can potentially be used to sequence long strands of DNA without labels or amplification, but a viable technique has yet to be established. Here, we show that single DNA molecules can be sequenced by monitoring the electrical conductance of a phi29 DNA polymerase as it incorporates unlabelled nucleotides into a template strand of DNA. The conductance of the polymerase is measured by attaching it to a protein transistor that consists of an antibody molecule (immunoglobulin G) bound to two gold nanoparticles, which are in turn connected to source and drain electrodes. The electrical conductance of the DNA polymerase exhibits well-separated plateaux that are similar to 3 pA in height. Each plateau corresponds to an individual base and is formed at a rate of similar to 22 nucleotides per second. Additional spikes appear on top of the plateaux and can be used to discriminate between the four different nucleotides. We also show that the sequencing platform works with a variety of DNA polymerases and can sequence difficult templates such as homopolymers.
URI: http://dx.doi.org/10.1038/NNANO.2013.71
http://hdl.handle.net/11536/144841
ISSN: 1748-3387
DOI: 10.1038/NNANO.2013.71
期刊: NATURE NANOTECHNOLOGY
Volume: 8
起始頁: 452
結束頁: 458
Appears in Collections:Articles