標題: Glucosamine-Based Supramolecular Nanotubes for Human Mesenchymal Cell Therapy
作者: Talloj, Satish Kumar
Cheng, Bill
Weng, Jen-Po
Lin, Hsin-Chieh
材料科學與工程學系
Department of Materials Science and Engineering
關鍵字: supramolecular chemistry;self-assembly;hydrogels;nanotubes;biocompatibility;wound healing
公開日期: 2-May-2018
摘要: Herein, we demonstrate an example of glucosamine-based supramolecular hydrogels that can be used for human mesenchymal cell therapy. We designed and synthesized a series of amino acid derivatives based on a strategy of capping D-glucosamine moiety at the C-terminus and fluorinated benzyl group at the N-terminus. From a systematic study on chemical structures, we discovered that the glucosamine-based supramolecular hydrogel [pentafluorobenzyl (PFB)-F-Glu] self-assembled with one-dimensional nanotubular structures at physiological pH. The self-assembly of a newly discovered PFB-F-Glu motif is attributed to the synergistic effect of pi-pi stacking and extensive intermolecular hydrogen bonding network in aqueous medium. Notably, PFB-F-Glu nanotubes are proven to be nontoxic to human mesenchymal stem cells (hMSCs) and have been shown to enhance hMSC proliferation while maintaining their pluripotency. Retaining of pluripotency capabilities provides potentially unlimited source of undifferentiated cells for the treatment of future cell therapies. Furthermore, hMSCs cultured on PFB-F-Glu are able to secrete paracrine factors that downregulate profibrotic gene expression in lipopolysaccharide-treated human skin fibroblasts, which demonstrates that PFB-F-Glu nanotubes have the potential to be used for wound healing applications. Overall, this article addresses the importance of chemical design to generate supramolecular biomaterials for stem cell therapy.
URI: http://dx.doi.org/10.1021/acsami.8b03226
http://hdl.handle.net/11536/144958
ISSN: 1944-8244
DOI: 10.1021/acsami.8b03226
期刊: ACS APPLIED MATERIALS & INTERFACES
Volume: 10
起始頁: 15079
結束頁: 15087
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