標題: Role of apamin-sensitive small conductance calcium-activated potassium currents in long-term cardiac memory in rabbits
作者: Yin, Dechun
Chen, Mu
Yang, Na
Wu, Adonis Z.
Xu, Dongzhu
Tsai, Wei-Chung
Yuan, Yuan
Tian, Zhipeng
Chan, Yi-Hsin
Shen, Changyu
Chen, Zhenhui
Lin, Shien-Fong
Weiss, James N.
Chen, Peng-Sheng
Everett, Thomas H.
分子醫學與生物工程研究所
Institute of Molecular Medicine and Bioengineering
關鍵字: Cardiac memory;Electrophysiology;Ion channel;Ventricular fibrillation
公開日期: 1-May-2018
摘要: BACKGROUND Apamin-sensitive small conductance calcium-activated K current (I-KAS) is up-regulated during ventricular pacing and masks short-term cardiac memory (CM). OBJECTIVE The purpose of this study was to determine the role of I-KAS in long-term CM. METHODS CM was created with 3-5 weeks of ventricular pacing and defined by a flat or inverted T wave off pacing. Epicardial optical mapping was performed in both paced and normal ventricles. Action potential duration (APD(80)) was determined during right atrial pacing. Ventricular stability was tested before and after I-KAS blockade. Four paced hearts and 4 normal hearts were used for western blotting and histology. RESULTS There were no significant differences in either echocardiographic parameters or fibrosis levels between groups. Apamin induced more APD(80) prolongation in CM than in normal ventricles (mean [95% confidence interval]: 9.6% [8.8%-10.5%] vs 3.1% [1.9%-4.3%]; P <.001). Apamin significantly lengthened APD(80) in the CM model at late activation sites, indicating significant I-KAS up-regulation at those sites. The CM model also had altered Ca2+ handling, with the 50% Ca2+ transient duration and amplitude increased at distal sites compared to a proximal site (near the pacing site). After apamin, the CM model had increased ventricular fibrillation (VF) inducibility (paced vs control: 33/40 (82.5%) vs 7/20 (35%); P < .001) and longer VF durations (124 vs 26 seconds; P < .001). CONCLUSION Chronic ventricular pacing increases Ca2+ transients at late activation sites, which activates I-KAS to maintain repolarization reserve. I-KAS blockade increases VF vulnerability in chronically paced rabbit ventricles.
URI: http://dx.doi.org/10.1016/j.hrthm.2018.01.016
http://hdl.handle.net/11536/144984
ISSN: 1547-5271
DOI: 10.1016/j.hrthm.2018.01.016
期刊: HEART RHYTHM
Volume: 15
起始頁: 761
結束頁: 769
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