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dc.contributor.authorHsu, Feng-Chunen_US
dc.contributor.authorSie, Yong Daen_US
dc.contributor.authorLai, Feng-Jieen_US
dc.contributor.authorChen, Shean-Jenen_US
dc.date.accessioned2018-08-21T05:56:24Z-
dc.date.available2018-08-21T05:56:24Z-
dc.date.issued2018-01-01en_US
dc.identifier.issn0277-786Xen_US
dc.identifier.urihttp://dx.doi.org/10.1117/12.2289336en_US
dc.identifier.urihttp://hdl.handle.net/11536/146157-
dc.description.abstractLight field technique at a single shot can get the whole volume image of observed sample. Therefore, the original frame rate of the optical system can be taken as the volumetric image rate. For dynamically imaging whole micron-scale biosample, a light field microscope with temporal focusing illumination has been developed. In the light field microscope, the f-number of the microlens array (MLA) is adopted to match that of the objective; hence, the subimages via adjacent lenslets do not overlay each other. A three-dimensional (3D) deconvolution algorithm is utilized to deblur the out-of-focusing part. Conventional light field microscopy (LFM) illuminates whole volume sample even non interesting parts; nevertheless, whole volume excitation causes even more damage on bio-sample and also increase the background noise from the out of range. Therefore, temporal focusing is integrated into the light field microscope for selecting the illumination volume. Herein, a slit on the back focal plane of the objective is utilized to control the axial excitation confinement for selecting the illumination volume. As a result, the developed light field microscope with the temporal focusing multiphoton illumination (TFMPI) can reconstruct 3D images within the selected volume, and the lateral resolution approaches to the theoretical value. Furthermore, the 3D Brownian motion of two-micron fluorescent beads is observed as the criterion of dynamic sample. With superior signal-to-noise ratio and less damage to tissue, the microscope is potential to provide volumetric imaging for vivo sample.en_US
dc.language.isoen_USen_US
dc.subjectlight fielden_US
dc.subjecttemporal focusingen_US
dc.titleVolumetric bioimaging based on light field microscopy with temporal focusing illuminationen_US
dc.typeProceedings Paperen_US
dc.identifier.doi10.1117/12.2289336en_US
dc.identifier.journalTHREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXVen_US
dc.citation.volume10499en_US
dc.contributor.department光電學院zh_TW
dc.contributor.departmentCollege of Photonicsen_US
dc.identifier.wosnumberWOS:000432542000022en_US
Appears in Collections:Conferences Paper